pcDNA3.1-3xFlag-CENPJ 小鼠CENPJ基因ORF克隆
ATGTTCCTGATGCCAACCTCTTCAGAATTAAACAGTGGGCAGAATTTCCTAACCCAGTGGATGACCAGTCCTTCTCGGGCTGGGGTCATACTAAATCGAGGATTTCCTATTTTGGAAGCAGATGATAAGCAAGCAGCTACAAATGTTTCTACGAGCTTCCCTGCTAAAGCCACCCATTTTTCAAATAGCTTCAGTATTAGTAGTGAAGAGGATTCATTTCATGAAGAACAGAAGTTGGAGGCTGGAGGACCTTATAAACCATGGTCAGAAAACCCTGAAGCTCCTCCGGTCTTTCCTTCAGTTAGAAAGGAGCCAATTGCATCACGTCAGGATGCCCCGGGATGCCAGGAGGATAACAACAATGATCTTACGCCTCATCTCGAGAGTGAATTCAAAGAAGTGGCTAATAAAAACCCACTTTTTAAAAAACTGGAACAGCTGAAAGAGATCCAGCAGAAGAAGCAGGAGCAGCTGAAGAGGCAGCAGCTGGAACAGCTGCAGAGGCTCATGGAGGAGCAAGAGAAGCTGCTCACCATGGTGTCTGCACAGCACGCGTTTCCAGGTACATTACTGCCTGATGATCAGAGCCAGAAGCATCGCTCTCCCGGAGATTTAACTCTGCCACCACACAGCTACTCAAATCCAACCCAAGAAAACTCCTGTGCTTCCAACGTTTTACCCGATGAACAGAGTAATTTCTGTAGAGCCACTCAAGATTCCGTATTAACTTCAAAAAATGCTTCGGATTTGTTTTACGAGTCACAGTATCAAGAAGCACATGTGAAAAGAAATGATTTAAAGGAGGAAAGTCCTGCCCACCCTTCAGGGGAAGGTGCTTTGCCACGTTGGGAAAAGAAGATGGGGAGGAGTCAGGAAGGGAAGGATGTGAACTTACAAAAATGTGGAGATTCTTCAGAAGTGGTTAATATTGATGAAAGGCCCATCAAAGCTGCTGTTAGAGAGAAGCAGCAGACATTTGAGGACTACTTAGAAGAGCAGATCCAACTGGAAGAGCGGGAGCTTAGACAGAAGCAGCTGCAGGAAGCAGAAGGACCATTGCTAGCGAAAACGAAACCCAAGCAACCATTTTTAAAACGAGGAGAAGGTTTAGCTAGGTTTACTAATGCTAAATCTAAATTTCAAAAGGGGAAAGAAAGTAAGCTAGCAAGTACGCAGAGCCCATCAGAGGACCAGCCTGGGTCTAAAGTAGACAGACAGCATTTGCAGAGGAAAACTGCTCTCATAAATAAAGACCTGTGTGCAGAAACCCCCACTGTTAAAAAGGACAGTAAAGCTAGACCCAAGGCTGGCTTTGCCTCACTCAGGCAGAAACCCAAAGTGACCAAGACCAATATGAGAGAAAGTCTCTCCCCACCAGGACTGAAAGTCCAGACGGGGAAGAAACGTGATGGACAGTTTAGACACCAGGTCAAAGGAGAACGAAATGCACATGCTAGTAATAAGGAAAATGTGCCTGCATGCATAAAGCCCTGGGATGCTGGTTGCAAGATGTGGAGTAAGACACAGGGGAGAGAGAGACTTCCTCTTTCCACTGGACCAGTTGGCTGTGTGGTCTCTAGGAGCCCGATACGTGAGACAGACAGAGAAACAGAATCTTCCCTTGATTTTTCTCTTCAGAAAAAATTAGAGATTTGGGAACGAGAAAAAGAAAAGGAAAACTTGGAATTAGATGAATTTTTATTTTTGGAACGGGCTGCTGATGAAATATCATTTTCTAGTAATTCCTCATTTGTACTGAGAATCTTAGAAAGGGACCAGCAGATCTGCGACGGTCACAGGCTGTCTTCTACTCCTGTCAAAGCTGTACAGCAGCGTGAGGCACAGCAGGCAGATCCTAGGGGTCAGAGTAACTGCAGTGAGATTCCCCGTTACGGTGTCGCACATGAGAACGAGAGTGAATGCGAGGCCATGCTCTTGTCTTGGGGTTCAGGGTCACCTGATGGTTTGAGGGAACTGTCCTGTAAACGTAGTATGAAAGCCTTCCAAACCAGCACTTCTGAGATCCAGAGCCAATGGGATGCAAGGGATGATGGCGTTGCCAACAGCGACAGTAGCACTGAGTCTGAGGAGCAGCATGACATTACCATCAAACCGTCCACCGAGGTTGGGGACAGGGTCTTCAGCAACAGAGAGGATAGTCCACAAGTCTGTGATGCCAAAGGACCTATTAGGGACACCGGGGCTCAGGAAGATAAATGGAGAGACGCAGACCTGGATCTGTCTGATAAAGAGTGTAGCAGTGACGAGTCTGTCATAGTTGAAAGCCTGAACAATAAAGTATTGGAACCACTGAGACTGCCCTCCTCTCAAGCTGGGAGTAAAATTGACTTCGATGATGAAAGATCGTGGACAGACCTTGAGGAGAATCCATACGAACATGGTGTTATCCACAGAGAGGAAGCCATATATGGTACACCCCAGACACAGTGCCACAGTAAGAGTGAAGGGTGTGTTCTGGACAAAACAATAAAAAGGAAGATTGCACCAGTCAAGAAGGGAGAAGACTTCAAGTGTGACAGGCGCATAAGCCCTCCTCCTCCATCTGACTTGATGGTAAAATTCTTCCCTTCTTTGAAACCAAAGCCAAAATTAGATTCACACTTGGAAAATGAATCCAAGTTAAACCTGAGTCAAGATCAACCACCAGAATTTATGGTCTGTTTTATAGGTGACAGTGTTCGATCTCAGGTTTTAAGAGAGAAAGTTACTGAATTGGAATCAGAAATAGAAAAATTTAAAGCTGAGAACACATCTTTAGCTAAACTTCGTATTGAACGAGAAAGTGCCTTGGAAAAACTGAGGAAAGAAATTGCTGACTTTGAGCAACAAAAAGCAAGAGAGCTGGCTCGAATAGAAGAGTATAGAAAGGAGGAGACAAGGAAACTACAAAAGGAGCGCAAAGTCTTTGAGAAGTACACAGCAGCTGCAAGAACATTCCCAGATAAAAAGGAGCGCGAAGAAATACAGGCCTTGAAACAGCAAATAGCAGATTTACAGGAAGACTTGAAAAGGAAGGAAACAAAGTGGTCAAGCACACAGAGTCGCCTCAGAAGCCAGATAGAGATGCTCGTCAAAGAGAACACAGACCTCAGGGAAGAGATAAAAGTAATGGAAAGGTTCCGACTGGATGCCTGGAAGAGAGCAGAAGCTATGGAGAACAGTCCCAAGGCCTGCCAGTACATGATGGCCACAAAAAAGGATGAGTCCATGAACTCATCATTTCAATTTCAAAAGAGTCATGTTTCTTCAGGAGTCCAGGTAGAAAAATATAAGAAAAAGTATTTGCCAGCACAAGGAAATCTATCTAGAAGAATCAAGTCTGCGCCTCCTCGTGATTTAGGCAGCTCCGATAAAGGACAAGCTGCCTTGCCCAGAGAGCCTCTTCAACAAGTGAACTTCCCAGACCTTGAATATAAAAACAAAGAGGAAAAAGAGGAAGAAATTCAGGGAGAAATCAGTCATCCTGATGGAAAGGTAGAAAAAATTTATAAAAATGGGCGCCGGGTTGTTCTGTTTCCCAATGGAACTCGGAAAGAAGTGAGTGCGGATGGGAAGAGCGTCACCGTTACATTTTTCAATGGTGATGTGAAGCAGGTCATGCCAGATGAGAGAGTGGTCTACTACTATGCTGCTGCCCAGACCACACACACCACGTACCCAGAAGGACTTGAAGTTTTACATTTCTCAAGTGGACAAATAGAAAAGCATTTCCCAGATGGAAGAAAAGAAATCACATTTCCTGACCAGACCATTAAGACTTTATTTGCTGATGGTCAAGAAGAGAGCATCTTTCCAGATGGCACAATTGTCCGAGTACAACGTGATGGCAACAAGATCATAGAATTTAATAATGGCCAAAGGGAGCTCCATACTGCCCAGTTCAAGAGACGAGAGTATCCCGATGGGACTGTTAAGACTGTCTATGCAAATGGTCATCAAGAGACAAAGTACACATCTGGCCGAGTTAGAGTGAAGGACAAAGACGGTAATGTGCTAATGGACACAGAAATGTGA
| 载体名称: | pcDNA3.1-3xFlag |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Neomycin / G418 |
| 5’测序引物: | CMV-F: CTAGAGAACCCACTGCTTAC |
| 3’测序引物: | BGH-R: TAGAAGGCACAGTCGAGG |
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
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