pEGFP-N1-OBSCN 小鼠OBSCN基因ORF克隆
ATGGACCACTCCTTCAGCGGAGCACCCCGCTTCCTGACGCGGCCAAAGGCCTTTGTGGTATCTGTTGGCAAGGATGCCACGCTGAGCTGCCAGATCGTGGGCAACCCCACGCCACACGTGAGCTGGGAGAAGGACCGGCAGCCAGTAGAGGCAGGAGCGCGCTTCCGCCTGGCCCAGGACGGGGATGTGTACCGCCTCACAATTCTCGATCTGGCGCTAGGTGACAGCGGGCAGTATGTGTGTCGCGCGAGGAACGCCATAGGGGAGGCCTTCGCCGCCGTCGGCCTGCGAGTGGACTCGGAGGGCACGTGTGCCGAGCAGGCGCCCCACTTTCTGCTGCGGCCCACATCCATTCGCGTGCGCGAGGGCGCAGACGCCACCTTCCGATGTCGCGTTGGAGGCTCACCACAACCTGCTGTGAGCTGGTCCAAAGATGGGCGGCGCCTGGGCCCACCCGATGCCCCCCATGTGCGTGTGGAAGAACACGGGGAGTCGAGCGCGCTTCGCATCCGTTCGGCAAGGCCTCGCGATGGTGGCACCTACGAAGTCCGAGCAGAAAATCCGCTGGGCTCTGCCAGCGCTGCCGCCGCTCTGGTGGTGGACTCGGACGCCGAGGTTGCCGGACCACCCGGCACCTCCACCGCCACGCTCCTGGCGCACCTGCAGCAGCGGCGGGAGGCCATGCGCGCAGAGGGCATACCCCCTTCTCCACCCGGCGCTGGCACGCGCACCTGCACGGTGACCGAAGGCAAACACGCGCGCCTCAGCTGTTTTGTGACCGGCGAGCCCAAGCCCGAGACAGTGTGGAAGAAGGACGGGCAGCTGGTGACCGAGGGCCGGCGACACGTAGTGTATGAGGACGAGCAAGAGAACTTCGTCCTTAAGATTCTTTTCTGCAAGCAGTCCGACCGCGGCCTCTACACCTGCACAGCGTCCAACCTCGTGGGCCAGACCTACAGCTCGGTGCTGGTGGTCGTGAGAGAGCCGACGGTGCCCTTCAAGAAGCGGCTGCAGGACCTGGAGGTACGAGAGAAAGAGTCTGCTACATTCCAGTGCGAGGTGGCTCAGCCAGCTACCGAGGCGGCGTGGTTCAAGGAGGAGACCCGGCTATGGGCCAGCGCCAAGTACGACATCGAGGAGGAGGGCACCGAGCGCCGGCTGACCGTGCGCAACGTCTCGGCAGACGACGACGCTGTGTACATCTGCGAGACCACAGAGGGCAGCCGCACGGTGGCAGAGCTCTCTGTTCAAGGAAACTTGACCAGAAAGCTGCCTCGGAAGACAGCAGTGCGCACTGGAGACACAGCCATCTTTTGGGTGGAGCTGGCTGTCCCCGAAGGCCCTGTCCGGTGGCTACGGAACCAGGAGGAGATGGTGGCAGGGGGTCGGGTTGCCATCACTGCAGAAGGCACGTGCCACACACTGACCATCTTCCAGTGCACCTTGGAGGACATGGGTGAGGTGGCCTTTGTGTCTGGTGGCTGCCGGACGACCACCCAGTTCTGTGTATCAGCGCCCAGGAGGCCACCCCTGTACCCTCCTGCTGACCCTGTTGTAAAGGCCAAGACAGAGAGTTCTGTGACCCTCAGCTGGTCCGCACCACCCCATGGGGACCGCCCTGTCACTATTGATGGCTATGTGTTGGAGAAGAGGAAGCTGGGTGCCTATGCCTGGAGCAGGTGTCACGAAGCTGGATGGCTGGCCACAACTGAGTTTACCATCACTGGTGTGGCTGAGGAGGGGGATTTCCAGTTCCGAGTGTCAGCCATCAATCACTTTGGCCAGGGTCCTTACCTTGAATTTCCAGGGACCATGCACTTGGTCCCCATGCTGGCTGTAAAGACACCTCTGAAGGCGGTGGAGGCAGTGGAGGGTGGTGAGGTCACTTTCTCCGTGGACCTCACGGTGGCCTCTTCGGGCGAATGGTTCCTGGATGGGGAGGCCTTGAAAGCGAGCAGTATATACGTGATCCGTTGTGACCGCACCCGGCATATGCTCACCATCAGAGAGGTGCCCGCCAGATTGCATGGGGCACAGCTGAAGTTTGTGGCTAATGGCATTGAAACTAGCATCCAAATGGTGGTCCGAGCGGCTCTGGGGCTGCCCAGCAGTAAGCTTCCTGCTGCGGCTGCCCGGGAGGTGCTGGCCCAGTTGCACGAGGAGGCACAGCTGCTGGCTGAGCTGTCGGACCAGGCTGCTGCTGTGACTTGGCTGAAGGATGGTCGTGAGCTGTCCCTAGGGCCCAAGTATGAGATGCAGGTGTCAGCTGGGAGGCGAGCACTGCTGGTGCGGGACGTGGCACAGGATGACGCTGGCCTCTATGAGTGTGTCAGTCGTGGGAGCCGCACTGCCTACCAGCTGTTGGTGCAAGAACCCAAGCTGGTGTTTGCCAAGGGGCAGCAGGCACACAGCGAGGTGAAGGCAGAGGCGGGGAACAGCGCCACCCTGAGCTGCGAGGTGACCCAGGCCCAGACTGAGGTGACATGGTTCAAGGATGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCATGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGACAGAAGCTCTCCTTCCGCCTGGACGTGGCAGAACCCAAGCTGGTATTTGCCAAGGGGCAGCAGGCACACAGTGAGGTGAAGGCAGAGGCGGGGGCCAGCGCCACACTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCATGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGACAGAAGCTCTCCTTCCGCCTGGACGTGGCAGAGCCCAAGATGGTGTTTGCCAAGGAGCAGCAGGCACGCAGCGAGGTGAAGGCCGAGGCGGGGGCCAGCGCCACACTGAGCTGCGAGGTGGCCCAGGCTCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCATGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCAGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGACAGAAGCTCTCCTTCCGCCTGGACGTCACAGAGCCCAAGCTGGTGTTTGCCAAGGAGCAGCAGGCACGCAGTGAGGTGAAGGCAGAGGTGGGGAACAGTGCCACACTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCATGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGGCAGAAACTCTCCTTCCGCCTGGACGTGGCAGAACCCAAGCTGGTGTTTGCCAAGGAGCAGCAGGCACGAAGTGAGGTGAAGGCAGAGGCAGGGAACAGTGCCACACTGAGCTGTGAGGTGGCCCAGGCACAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCAAAGGTGCGCATGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGACAGAAGCTCTCATTCCATCTGGACGTCACAGAACCCAAGCTGGTGTTTGCCAAGGAGCAGCAGGCACACAGTGAGGTGAAGGCAGAGGCGGGGGCCAGTGCCACCCTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGAAAGAAGCTGAGCTCCAGCTCAAAGGTGCGCATGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGAGGGACAGAAGCTCTCCTTCCGCCTGGACGTGGCAGAGCCCAAGCTGGTGTTTGCCAAGGAGCAGCAGGCACGCAGCGAGGTGAAGGCAGAGGCTGGGGCCAGTGCCACACTGAGCTGTGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGAGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGCCAGAAGCTCTCCTTCCGCCTGGACGTCGCAGAGCCCAAGCTGGTGTTTGCCAAGGAACAGCAGGCAAACAGCGAGGTGAAGGCCGAGGCGGGGGCCAGCGCCACACTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGGCAGAAGCTTTCCTTCCGCCTGGACGTGGCAGAGCCCAAGCTGGCGTTTGCCAAGGAGCAGCAGGCACACAGCGAAGTGAAGGCAGAGGCGGGGGCCAGCGCCACACTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGATGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGACAGAAGCTCTCATTCCGCCTGGACGTGGCAGAGCCCAAGCTGGCGTTTGCCAAGGAGCAGCAGGCACACAGCGAGGTGAAGGCAGAGGCGGGGGCCAGCGCCACACTGAGCTGTGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGTCAGAAGCTCTCCTTCCGCCTGGACGTGGCAGAGCCTAAGCTGGCGTTTGCCAAGGAACAGCAGGCACACAGCGAGGTGAAGGCAGAGGCGGGGGCCAGTGCCACACTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGACAGAAGCTCTTCTTCCGCCTGGATGTGGCAGAGCCCAAGCTGATGTTTGCCAAGGAGCAGCAGGCACACAGCGAGGTGAAGGCAGAGGCTGGGGCCAGCGCCACACTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGGCAGAAGCTCTCCTTCCGCCTGGATGTGGCAGAGCCCAAGCTGGTGTTTGCCAAGGAGCAGCAGGCACACAGTGAGGTGAAGGCCGAGGCGGGGGCCAGCGCCACCCTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGTGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCAGGGGACAGAAGCTCTCCTTCCGCCTGGACGTGGCAGACACCAGGCTGATGTTTGCCAAGGAGCAGCAGGCACGCACCGAGGTGAAGGCTGAGGCGGGGAACAGTGCCACCCTGAGCTGCGAGGTGGCCCAGGCCCAGACTGAGGTGACATGGTTCAAGGACGGGAAGAAGCTGAGCTCCAGCTCGAAGGTGCGCGTGGAGGCCTCGGGCTGCTCCAGGAGGCTGGTGGTGCAGCAGGCGGGCAAGGCGGATGCTGGGGAGTACAGCTGCGAGGCCGGGGGTCAGAAGCTCTCCTTCCGCCTGGACGTGGCAGAGGCCGAATCCCAGATTCCGGAGAGACCCAGCCGCAGGGAACCTCTGGTTGTCAAGGAACATGAGACCATTATCCTGACTGCCACAATAGCTGCACCCTCTGTGGCTGCTGTGACCTGGCTCAAAGATGGAGTGGAGATCCGCCGCAGTAAGCGGCATGAGGCCACCAGTCTGGGTGATACCCATACCCTGACCGTGAGAGGCGCACAGGTCCTGGACAGTGCCATCTACAGCTGCCGTGTTGGCAAGGAAGGTCAGGACTTCCCGGTGCAGGTAGAAGAGGTGGCCGCCAAGTTCTCCAAACCCCTGGAGCCCGTCGAAGGGGAGCTGGGTGGCACCGTGATGCTGGTTTGTGAGCTGAGCCCGGAGCAGGCTGAGGTTGTGTGGCGCTGTGGGAACACACAGCTGCGGCCAGGCAAGCGCTTCCAGATGACATCAGAGGGGCCTAGGCGCACGCTGACCGTGTCTGGCCTTCGAGAGGATGACGCAGAGGAGTATGTGTGTGAGAGCCGGGATGACCGCACCAGCGCACGGCTCACTGTTAAAGTTCCCCGAGTGGTCAAGTTTACGTCTGGATTGAGTGCCATGGTCGCAGAGGAGGGCCAAGAGGCCACCTTTCAGTGTGTCGTGTCCCCCAGCGATGCAGGGGTCACATGGTACAAGGACGGGATGCAGCTGCAGCCTAGCGAGAAGTTTGTTATGGTAGAGAGTGGGGCCAGCCGCAGCTTGACTATCTTGGGCCTGACCCTGGAGGATGCCGGTCAAGTCACAGTGGAGGCTGAGGGGGCTTCATCTTCTGCTGCCCTCCGGGTCCGAGAGGCACCGGTCCTCTTCAAAAAGAAGCTGGAGCCACAGACAGTGGAGGAGAGGACCTCTGTGACATTGGAGGTGGAGCTGACGCGGCCCTGGCCCGAGGTGAAGTGGACACGGAATGCTGCCGTCCTGACACCCAGCGAGAACGTGGAGATCCGCGCTGAGGGTGCTCGGCACTGTCTGGTGCTGCGCAGCGTGGGCTTTGCTGACCGCGGCTTCTTTGGCTGTGAGACACCAGATGATAAGACTCAGGCCAAGCTCAATGTAGAAATGCGGCAGGTCCGGCTGGTTCGCGGTCTGCAGGAGGTGGAGGCTAAGGAACAGGGCACGGCCTCGATGGATGTGGAGCTGTCCCATGCCGAGGTGGAGGGCAGCTGGACCCGAGATGGCCTGCGACTTCAGCCTGGACCCAAATGCCACCTGGCCGTACAAGGCCCTGTCCACATCCTCACACTCTCGGCGCTGCAGCCACAGGACAGTGGGCTGGTGGCCTTCAGGGCCGAGGGCGTGCACACGTCTGCGAGGCTCATAGTCACTGAGTTGCCCGTGAGCTTCACCAGAGTACTACAGGATGTGGTGGCCACGCAGAAGGAGAAGGTCACCCTGGAGTGTGAGCTGTCGAGGCCTGTGGATGTGCGCTGGCTGAAGGATGGTGTGGAGCTGCGGGCAGGCAAGGCCATAGGCATAGTGGCCCAGGGAACCTGCAGGAGTCTCGTTATCTACCGCTGTGAGACTGGGGACCAGGGTGTCTATGTATGTGATGCTCTTGATGCTCAGACCTCTGCCTCTCTGAGGGTGCAAGGGAGGACATACACGCTTATCTTCCGGAGGGTACTGGCAGAGGATGCAGGGGAGGTCAAGTTTGTAGCTGAGAACGCAGAATCAAGAGCTCACCTCCGAGTGAAAGAACTGCCCGTGACCCTCCTGCGCCCACTGAGGGACAAGATTGCCATGGAAAAACACCGTGGAGTGCTGGAGTGCCAGGTGTCCCGGGCTAGTGCCCAGGTGCGGTGGTTCAAGGGCGGAGTCGAGCTGCAGTCTGGGCCCAAGTACGAGGTGGTCAGTGACGGCCTTTACCGCAAGCTGGTCATTAATGACGTGCAGCCTGAGGATGAAGATACGTACACCTGTGACGCTGGCAACGTCAAGACCAGTGCCCAGTTCTTTGTGGAAGAGCAATCCATCACTATTGTGCGGGGACTGAAGGACATGACAGTGATGGAGCCTGCCCCTGCCTGGTTTGAATGTGAGACATCCATCCCTTCCGTGAGGCCACCCAAGTGGCTCCTTGGTAAGACTGTGCTCCAGGCAGGGGGGAACGTGGGACTGGAGCAAGACGGCACCGTGCACCGGCTGACACTGCACAAGACCTGCTCTACCATGACCGGGCCTGTGCACTTCACTATCGGCAAGTCCCGCTCCTCTGCCCAGCTGGTTGTCTCAGACATTCCTGTGGTACTGACACGGCCTCTGGAGCCCAAAGCAGGGCGTGAACTGCAGTCAGTAGTCCTGTCCTGTGACTTCAGGCCAGCCCCCAAGGCTGTGCAGTGGTACAAGGATGACACACCCCTGTCCCCGTCAGAAAAGTTCAAGATGGCGCTGGAGGGCCAGATGGCAGAGCTGCGCATACTCCGGCTCACGCCAGCTGATGCTGGGGTCTACCGGTGCCAGGCGGGTAGCGCCCAGAGCAGTGCTGAGGTTACTGTGGAAGCTCGGGAGGTGACAGTGATTCAGCCACTGCAGGATGCAGAAGCCATGGAGGAAGGCCGGGTCTGCTTCTCCTGCGAGCTGTCCCATAAGGATGAAGATATCGAATGGTCACTCAATGGTACACCCCTGTACAATGACAGCTTCCATGAGATCAGCCATGAGGGCTGTCTCCACACCCTGGTGCTGAAGAGTGTCCGGCAGGCTGACACAGGCACCGTGTGCGCCACCTCCCCCAAGGTGTCCGTTTCCGCCCGGCTGGTGGTTAAAGGCAAGCCAGTGGTGTTCCTGAAAGCACTGGATGACGTATCTGCAGAAGAGCGTGGCACGCTGACCCTGCAATGCGAGGTCTCCGACCCCGAGGCACGCGTGGTTTGGCGCAAAGATGGTGTGGAGCTTGGTCCCAGCGACAAGTATGATTTCCTACATAAGGCAGGTGCCCGGGGCCTTACGGTCCATGACCTGAGCCATGAGGATGCTGGACTGTACACCTGCCAGGTGGGCAGTAAGGAGACCCAGTCCAAGGTCAGCGTACATGATCTTCACGTGGGCATCACCAAGAGGTTAAAGACAGTGGAGGTGCTGGAGGGAGAGAGCTGCAGCTTTGAGTGTGTCCTTTCCCACGAGAGTCCCAGTGACCCAGCAGTGTGGACGGTCGGTGGGAAGACAGTGGGTGGTTCTGGCCACTTTCACGCCGTTCGGCAGGGCCGCAAATACACCCTGACAGTCAAAGATGCTGCACTCAGTGATGCAGGAGAGGTGGTCTTCTCGGTGCTGGGCCTCACTTCCAAGGCCTCGCTCATCATCAGAGAGAGGCCAGTGGACATCACCAAGCCCCTGGAGGACCAGCGGACGACGCTTGGGGAAGATGTAATGCTAAGCTGTGAGCTGTCCAGGGCAGGCACCTCTGTGCGCTGGCTGAAAGACGGGAAGGCCATCAGGAAGAGCCAGAAGTATGACCTGCTCAGTGAAGGCACGCGGGCTGTGTTGGTTGTCCGCAAGGCCTCACTCAAGGATTCAGGGGAATACACCTGCGAGACAGAAGCTTCGAAAAGCACTGCCAAGCTCTGTGTGGAAGAAAAGGCAAACCGGTTCACGGAGGAGCTGGCTGACCTGCAGGTGGAAGAGAAGGGCAGAGCTGTGTTCACATGCAAGACAGAGCACCCAGCATCTGTCGTGACCTGGCGCAAAGGCCTCCTGGAGCTGCGCGCCTCAGGGAAGCATGTCCCCAGTCAGGAAGGCTTGACCCTGAAGCTCACCATCAACGCCCTGGAGAGGACAGACAGTGACACTTACACCTGTGACATCGGCCAAGCCCGGACCCAGGCACGGCTCCTCGTCCACGGCCAGAAAGTGCGCGTTATTGAGGACCTAGAGGACACTGCTGTCCAGGAGGGCTCCTCTGCTAAGTTTTGCTGCCGCATCGCTCCTGCTGACTACGGCCCCGTGCACTGGTTCCTGGATAAGACCCCCTTGCACAGCAATGAGCTGAATGAGATCACTGTCCAGCCTGGAGGCTATCACGTGCTCACCCTGCGGCAGCTGGCACTCAAGGACTCAGGCACTGTCTACTTTGAGGCGGGTGACCAGCGAACCTCAGCTGCCCTGCGGGTGACTGAGAAGCCGAGCATCTTCTCTCGGCCACTCACAGACGTCACAGTCACAGAAGGCGAGGACCTGACTCTGGTCTGTGAAACCACCACTGTGGATAGCTCTGTGCGCTGGACCAAAGATGGGAAGACACTGAGGCCGTCTGCACGATGCCAGCTGAGCCATGAAGGCTGTCAGGCCCAGCTGCTCATCACTGCCACCACCCCACAAGATGGTGGGCGGTACAAATGTGAGATTGGTGGAGCCTCCAGCAGCTCCATTGTCAGGGTCCATGCTCTGCCAGTGCGGTTCAGGGAGTCCCTGAAGGACGTGGAGGTACCAGAGGGCAAGGCCGCCACACTGCGCTGTGTGCTGTCATCCGTGGCTGCACCTGTGGAGTGGCGTCATGGAGATGATGTCCTGAAATCCAGCAACAAGTATAGCCTGCGCCAAGAGGGTGCTGTGCTGGAATTGGTCATCCGAGACCTGCAGCCCCAGGACAGTGGGCAATACTCATGCTCCTTTGGGGACCAGACAACTTCAGCCACACTCACAGTGAAAACCTCTTCTGCCCAGTTCGTAGGGAAACTGAGAAACAAGGAGGCCACAGAAGGGACCACGGTCACACTTCGGTGTGAGCTGACCAAAGAGGCCCCCGTGGAGTGGAAGAAGGGAACAGAGACCCTGAGAAATGGGGACAAATACAGTCTGAAGCAGGATGGAGCTGTGTGTGAGCTGCAGATCTGTAGTCTGCTGGTGGCAGATGCAGGGGAGTACTCGTGTGTGTGTGGGCAGGAGAAGACTTCAGCCACGCTGACTGTCAAGGCTCTGCTTGTCCACTTTGTCAGAAGGCTCAGGAGCAAGGAGGCCACAGAAGGAGACACGACCACACTGCAGTGTGAACTGAGCAAGGCTGCCCCCGTGGAGTGGAGGAAGGGAACAGAGACCCTGAGAGATGGGGACAGATACAGCCTGAAGCAGGATGGGGCTGTGTGTGAGCTGCAAATCCGCAGCCTGACCATAGCAGATGCTGGCGAGTACTTGTGCACGTGTGGACAGGAAAAGACCTCAGCCACGCTGACCGTCAGGGCCCTTCCAGCTAAGTTCAAAGACAGTCTGAAGAATGAAGAGGCTACAGAAGGCACCACAGCTACACTGAGCTGTGAACTGAGCAAGGCAGCTCCGGTGACATGGAAGAAAGGACCGAAGACCTTGCAAAGTGGAGACAAATATGTCCTGAGGCAGGATGGAGCTGTGTGTGGACTACAGATCCATGGCCTGACCATGGCTGATGCTGGGGAGTACTCGTGTGTGTGTGGGCAGGAGAAGACGTCAGCCACGCTGACTGTCAGGGGCTTGCCTGCCAAATTTATAGAAGATCTGAGAAGCCAAGAAGCCACAGAAGGGGCCACAGCAATCTTAAGATGTGAGCTGAGCAAGGCTGCTCCTGTGGAGTGGAGGAAAGGGTCTGAGACCCTGAAAGATGGGGACAGATACACCCTGAGGCAGGATGGGGCCGTATGTGAGCTGCAGATCCGTGGCCTGGCTGTGGTGGATACTGGGACATACTCGTCCCTGCCCACCAAGTTCACAGAGGGTCTGAGGAATGAAGAGGCCACGGAAGGCACCATGGCCACTCTGAGGTGCCAGATGAGCAAGGCTGCCCCTGTGGAGTGGAGGAAGGGGTCAGAGACCCTGAGAGATGGGGACAGATACAGCCTGAGGCAGGATGGGGCCATGTGTGAGCTGCAGATCCGTGGCCTGACCATAGAAGATTCTGGGGAGTACACATGTGTGTGTGGGCAGGAGAAGACGTCAGCCACACTGAGTGTCAAGGCCCTGCCCTCCAGATTCATAGAAGATTTGAGAAGTCAAGAGGCCACAGAAGGCACCATGGCCACTCTGAGGTGCCAGATGAGCAAGACTGCCCCTGTGGAGTGGAAGAAGGGGTCTGAGACCCTGAGAGATGGGGGCAGATACAGCCTGAGGCAGGATGGGCCTGTGTGTGAGCTGCAGATCTGTGACCTGGCTGTCGAAGACGCTGGGGAGTACTCATGTGTGTGTGGGCAGGAGAAGACCTCAGCCACACTGAGTATCAAGGCTCTGCCCCCCAGATTCATAGAAGACTTGAGAAGCCAAGAGGCCACAGAAGGCACCATGGCCACTCTGAGGTGCCAGATGAGCAAGGCAGCCCCTGTGGAGTGGAGGAAGGGGTCTGAAACCCTGGGAGATGGGGGCAGATACAGCCTGAGGCAGAATGGTGCTGTGTGTGAGCTGCAGATCCATGACCTGGCTGTGGAAGACACTGGGGAGTACTCATGTGTGTGTGGGCAGGAGAAGACCTCAGCCACACTGAATGTCAAGGCCCTGCCCCCCAGATTCATAGAAGACTTGAGAAGCCAAGAGGCCACAGAAGGCACCATGGCCACTCTGAGGTGCCAGATGAGCAAGGCAGCCCCTGTGGAGTGGAGGAAGGGGTCTGAGACCCTGAGAGATGGGGGCAGATACAGCCTGAGGCAGGATGGGGCTGTGTGTGAGCTGCAGATCCATGACCTGGATGTGGAAGATGCTGGCCAGTACTCGTGCGTGTGTGGGCAGGAGAAGACCTCAGCCGTGCTGACTGTAGATGCACTGCCCCCCAAGTTTACAGAGGGTCTGAAGAAGGAAGAGGCCACAGAAGGGACCATGGTAACACTGAGGTGCCAGATGAGCAAAGAAGCCACGGTGGAGTGGAGGAAGGGAGCAAAGACCCTCAGCGATGGGGGCAGATACAGCCTGAGGCAGGATGGGGCCATGTGTGAGCTGCAGATCTGTGGCCTGGCTGTGGAAGACGCTGGGGAATACTCATGTGTGTGTGGGCAGGAGAAGACCTCAGCCACACTGAGTGTCAAGGCCCTGCCCCCCAGATTCATAGAAGACTTGAGAAGCCAAGAGGCCACAGAAGGCACCATGGCCACTCTGAGGTGCCAGATGAGCAAGGCTGCCCCTGTGGAGTGGAGGAAGGGGTCTGAGACCCTGAGAGATGGGGACAGATACAGCCTGAGGCAGGATGGGGCTGTGTGTGAGCTGCAGATCCGTGACCTGGCTGTGGAAGATGCTGGCGAGTACTTGTGCGTGTGTGGGCAGGAGAAGACCTCAGCCACACTAAGTGTCAAGGCCCTGCCCCCCAGATTCATAGAAGACTTGAGAAGCCAAGAGGCCACAGAAGGCACCATGGCCACTCTGAGGTGCCAGATGAGCAAGGCTGCCCCTGTGGAGTGGAGGAAGGGATCCAAGACCCTGAGAGATGGGGACAGATACAGCCTGAGGCAGGATGGGGCCATGTGTGAGCTGCAGATCTGTGACCTGGCTGTGGAAGACACTGGGGACTACTCATGTGTGTGTGGGCAGGAGAAGACCTCAGCCACACTGAGTGTCAAGGCCCTGCCCCCTAGATTCATAGAAGATTTGAGAAGCCAAGAGGCCAGGGAAGGTACTGTGGCCACTCTGAGGTGCCGGATGAGCAAGGCTGCCCCAGTGGAGTGGAGGAAGGGGTCAGAAACCCTGAAAGATGGGGACAGATACAGCCTGAGGCAGGAGGGGAACCTGTGTGAGCTGCAGATCCGTGACCTGGCTGTCGAAGACACTGAGGAGTACTCATGTGTGTGTGGGCAGGAGAAGACCTCAGCCACACTGAGTGTCAAGGCCTTGCCAGCCAAATTCATAGAAGACTTGAGAAGCCAAGAGGCCCCGGAAAGTTCAACAGTCACATTGCGGTGTAAGCTAAGCAAGAAGGCCTCTGTGGTGTGGAAGAAAGGGTCTGAGACCCTAAGAAATGGGGCCAGATACAGCCTGAGGCAAGATGGGGCTGTGTGTGAGCTGGAGATCCGAGACCTGACTGTGGAAGATACTGGAGAGTACTCGTGCACATGTGGGCAGGAGAGGACCTCAGCCACACTGAGCATTATGGCTCCACAGGTGGTATTCCAGCAGCCACTGCAGAATCTGCAAGCAGAAGAAGGCTCCATGGCCAGCCTGCGGTGTGAGTTGTCAGTCCCCAACGCTGCCATGGTCTGGAGCAAGGGTGGTCTGGAGCTGCAGGGTGACACGCGCCGAGAGGCACGGCAGCAGGGGTGTGTGGCAGAGCTGTTGCTTAGGGACCTGCGTCGCGAGGATGCGGGCGAGTACAGTTGCACCTGTGGCTCTCAGACCACAAGCGCCACGCTCATGGTCACAGCTGCTCCTGTGCGGTTCCTCCGGGAACTGCAGGCCCAGGACGTAGATGAGGGGGCCACCGCACGCCTGCGCTGTGAGCTGAGCCGGGAGGCTGTGAGCGTGGAGTGGCGCAAGGGCTCCTTGCAACTTTTTCCTTGTGCTAAGTATCAGATGGTGCAGGAGGGCACAACTGCGGAGCTGCTGGTCCATGGGGTGGAGCAGGAGGATGCAGGCGAATACACCTGCGATGCAGGCCACACGCAGAGCATTGCCAGGCTCTCTGTCCGAGCCCCCAAGCCCAAGTTCAAGACCGACCTGCAAAGCACAGAGCAGGAAGCAGGTGGCACGGCACGGCTGTGTTGTCAACTGAGCGAAGCTGAGCCAGGGACTCCAGTGCAGTGGCTCAAGGAGGGGGTGGAGCTGCACGTCGGCTCCAAGTATGAGATGCGGCGCCAAGGGGCTGTGTGCGAGCTGCTGATCCATGGGCTGGAGGCCAAGGACACTGGAGAATATGCCTGCCTGGTGGGTGGCCAGAAGACCTTGGCCTCCCTCAGAGTCAAAGAGCCTGAGGTGACCATCGTGCGGGGACTCGTGGACATGGAGGTGCAGGCCGATGAGGATGTGGAGTTCACTTGCAAGGTGTCACAGGCAGGAGCCACAGACGTGCAGTGGCACCTCCAAGGTCTGCCACTGCAGAGCAATGAAGTGACAGAGGTGGCTGTCCTTGCGGACGGCTGTACCCATGTGCTCCAGTTAAAGGGTGTGACACTGGAGGATGCTGGTACTGTCTCCTTCCACGTGGGCGGCCTTTCATCTTCTGCTCAGCTCACAGTCCGAGTCCCTGAGGTGACCGTTCTGGAGCCCCTGAAGGATGTGCAGCTCAGCGAGGGCCAGGATGCCCACTTCCGGTGCCGACTGTCCAGGGCCTCGGGCCAGGAGGCTCGCTGGGCTTTGGGAGGGGTTCCCTTGCAGTGCAACGAGATGAACGACATCACTGTGGAGCAGGGCACACTCTACCTGCTCACTCTGCACAAGGTGACCCTGGAGGATGCTGGGACCATCACTCTCCAAGTGGGCTCATGCTCCTCAGAGGCCCAGCTGAAGGTCACAGCCAAGAACACGGTGTTGCGTGGCCTGGAGAACGTGGATGCACTGGAGGGCGGTGAGGCTCTGTTCGAGTGCCAGCTGTCCCAGCCTGAAGTGGCCGCCCACACCTGGCTGCTGGACGACGAGCCTGTGCACACGTCGGAGAAGGTGGAGGTGGTCTACTTTGAGAACGGACTGCGGCACCTGCTTCTACTCAAAAACCTGAAGCCACAGGACAGCTGCCGAGTGACCTTCCTGGCAGGGGATGTGGTCACGTCCGCATTCCTCACTGTGAGAGGCTGGCGCTTGGAGGTCCTAGAGCCCCCACATGATGCATCTGTGAAAGCTGGTATGCAGGTCCGCTTCACATGCATACTAAGTGAGGCGGTGCCTGTGGGTGAGGCTACTTGGTACATCAACGGGGCTGCCATCCAACCTGATGACACTGACTGGACAGTCACCACAGATGGCAGCCACCATGCCCTGACGCTGAGCAATGCCCAGCCCCAACACGCAGGAGAGGTCACATTTGCAGCACGGGATGCTGTGGCCTCTGCACGCCTCTCTGTGTTGGCTCTTCCTGACCCCCCTGAAGATGCTGAAGTGGTGGGGCGTAGTGACCATTCTGTGACCCTATCCTGGGTGGCTCCCATGAGTGATGGAGGTGGGGGTCTATGTGGTTATCGTGTGGAGATGAAGGAGGCATCCACAGGCCAGTGGCAGTTGTGCCATGACTTGGTACCTGGTCCAGAGTGCGTGGTGGACGACCTGGTCCCCGGGAAGACCTACCGCTTCCGAGTGGCAGCTGTGGGTCCAGCAGGTGCTGGGGAGCCTGTACACTTGCCCCAGATGGTCAAGATAGCCCCAGCCCCGGCCCCGGCCCCGGCCCCTGCCCCAGCCCCTGCCCCAGAAACACGGCAGGCAGTAGTTGGTGAAGACATCTGTCTGGAGTTGGAAGTGGCAGCTGATGGTGGCGAGGTCGTCTGGCACAAGGGAACAGAGCGCATCCAGCCCGGAGGGCACTTTGAGGTCCTCTCTCGGGGTCAGCGGCAGATGCTGGTGATCAAGGGCTTTAGGACCGAAGACCAAGGGGAGTACCGCTGTGGTCCCATTCAGGGTCTGCCCTCCTCAGGAGCTGCCACGTTCAATGTGGTCATGACCTCAGGCTCTGGGGATGAGGTCCCGGCACAGCCTAGCCTGCCTCCCGAGGCAGCCCAGGAGGGTGACCTGCATCTGCTTTGGGAGGCCCTTGCCCGGAAGCGCCGCATGAGTCGGGAGCCCACGCTGGACTCCATCAGTGAGCTGCCTGAGGAAGACAGCCGTGTGCAGCATCTGCGGCAGGAGGCAGAAGAGACAGCTCCCGACCTCTCTGAAGGCTACTCTACAGCGGATGAGCTAGCACGCACAGGAGAGGCCGACCTCTCACACACCAGCTCTGACGATGAGTCTCGGGCTGGCACCCCTTCCCTAGTTACCTACCTCAAGAAGGCCGGGGGTCCTGGGATCTCACCCTTGGCCAGCAAGCATGAGGCCCAGGTGACCACGTCTGTGAAGCCACAAAAGCAGCAGGAGCCAGTCGTGCCCACGTGCCCACCGCCGGGAGACTTGAGTGCAGCAGATTTGATGGATCCATCCCTGGACAAGGCTGCTGTGAAGATTCAGGCTGCCTTTAAGGGTTACAAAGTGCGGAAGGAGATGAAACAGCAGGAAGGGCCTGTGTTCTCACGAACATTTGGGGACACAGAGGCACAGGTTGGGGATGTGCTGCGGCTAGAGTGTGTGCTGGCCACCAAGACTGATATGCGGGCCTGCTGGTTGAAGGATGGCATCGAGTTAACAGATGGGCGCCATTACCACATAGACCAGCTCAAGGATGGTACCTGCTCTCTGCTGGTCACTGGCCTGGCCCCCACTGACTCTGGCCGTTACACCTGTCAGGTGAGCACCAAGTCTGGCCGTGTGAGCCACAGTGCCTGCGTGGTGGTCAGTGGGACAGAGAGCGAGGCCGAGAGCTCCTCTGGGGGCGAGCTGGATGATGCTTTCCGCCGGGCAGCGAGACGCCTGCACCGCCTCTTCCGCACCAAGAGCCCAGCTGAGCTTTCAGAGGAGGAACTCTTCCTCAGCGCTGATGAAGGCCCCGGGGAGCCAGAGGAGCCCGCAGACTGGCAAACATACCGAGAGGATGAGAACTTTGTGTGCATCCGTTTTGAGTCCCTTGCAGAGGCCCGCCAGGCAGTCACCTGCTTCCGAAACATGTTTGCCACTATGGGCATCGGGGTGGAGATCAGCCTAGGGGAGCAGGGACCCCGGGGAGTGGAGATGCGCATCGGCAAGGTGGCCCCTACTGTCACCCCTGCAGTGCCGCTTGCAAAGACACCTGGCCTGCAGACTTCAGATGCTGCCCCAGTGTTCCTGACCGAGCTGCAGAACCAAGATGTGCAGGATGGATACCCCATGAGCTTCGACTGCGTGGTAACGGGCCAGCCTGTGCCCAGTGTGCGCTGGTTCAAGGATGGGAAGCTGCTAGAAGAGGATGACCACTATATGATCAATGAGGACCAACAGGGGGGTCACCAGCTCATCATCACAGCTGTGGTGCCGGCAGACATGGGTGTCTACCGCTGCCTGGCGGAGAACAGCATGGGCGTCTCCTCCACCAAGGCTGAGCTTCGTGTGGAATTGACCAGCACAGACTATGATACTGCTGCGGATGCTACTGAGACCTCATCCTACTTCAGCGCCCAGGGATACCTGTCCAGTCGGGAGCAAGAGGGAACGGAGTCAGATGAGGGGCAGCTTCCCCAGGTGCTGGAGGAACTGAAAGACCTCCAAGTGGCCCCCGGGACACGCCTGGCCAAGTTCCAGCTCAAGGTGAAAGGCTATCCAGCTCCCAAACTGTATTGGTTCAAAGATGGCCAGCCCCTGACCACGTCTGACCACATCCGCATGACTGACAAGAAGACCCTGCACACCCTGGAGATTGTCTCGGTCACTCGGGAGGACAGCGGCCAGTATGCAGCCTACATCAGCAATGCTGTGGGTGCTGCCTATTCGTCAGCCCGGCTTCTGGTCCGAGGTCCCAGTGAACCAGAAGAGAAGCCAGCATCAGATGTTCATGAGAGGCTGGTGCCACCCCGAATCCTGGAGAAGTTCACCCCCAAGAAAGTGAAGAGGGGCTCCAGCATCACCTTTTCAGTGAAGGTGGAAGGACACCCGGCCCCCAGTGTGCATTGGCTCAAGGAGGAGGCAGAGAAGGGAGTCCTGTGGATTGGCCCTGACACCCCGGGCTACACCATGGCCAGCTCTTCCAAGCAGCATAGCCTGGTCCTGCTGGACGTAGGCCGACAGCACCAGGGCACCTACACATGTATTGCTACCAACCCTGCTGGCCAGGCGCTCTGCTCTGCCAGCCTGCACATCTCTGGCTTGGCCAAAGAAGAAGAGCAGGAGAGAGTGAAGGAGGCCCTCATTTCCTCTTTCCTGCAAGGGACAAGCCAAGCTGTCTCAGCCCAGATGTCAGAATCCGCAGGTTTTGCTGATCTTGTCGGGCAAAGCAAAGGCGAGTCTCTGGTGGCTGAGGAGGCCCACAGTCACCTGTCCCTTGCTGAGGTGGGCACTGAAGAGTTCCTGCAGAAACTCACCTCGCAGATCACGGAGATGGTATCGGCCAAGATCTCTCAGGCCAAACTCCAGGTGCCTGGAGGTGACAGCGATGAAGAGACCAAGACACCATCTGCTTCTCCTCGGCATGGCCGGTCACGTCCTTCCTCCAGTGTTCAAGAGTCCTCCTCAGAGTCAGAGGATGGGGACTCCCGTGGTGAGATCTTTGACATCTATGTGGTCACAGCTGACTATCTGCCACTGGGAGCTGAGCAGGATGCCATCATTCTGAGAGAAGGCCAGTATGTGGAGGTCCTTGACTCTGCCCATCCCCTGCGCTGGCTTGTCCGGACCAAGCCCACCAAATCCAGTCCTTCCAGGCAGGGCTGGGTGTCACCTGCCTACCTGGATAAGAGGCTCAAGCTGTCTCCTGAGTGGGGGCCCACTGAGGCCCCCGAGTTCCCTGGCGAGGCTGTGTCTGAGGATGAGTATAGAACGAGGCTGAGCTCTGTCATCCAGGAGTTGCTGAGTTCAGAGCAGGCTTTTGTGGGTGAGCTGCAGTTCTTGGAGAGCCACCACATGAAGCACCTGGAGCGATCTCCCCGAGTACCCGCAGCTGTGGCCAGCCAGAAGACAGTCATCTTTCGTAATGTGCAGGACATCAGCCATTTCCACAGCAGCTTCTTGAAGGAGCTGCAGGGCTGTGGCACCGACGATGATGTGGCCATGTGCTTCATCAAGAACCAGGAGGCCTTTGAGAAGTACCTCGAGTTCCTGGTGGGCCGCGTGCAAGCTGAATCAGTGGTTGTCAGCACCCCAGTCCAGGAGTTCTACAAGAAATATGCAGAAGAGACACTGTCAGCCAAGGACCCCACACAGCCACCCCCACCTCCTCTTCAGCACTACCTGGAGCAGCCAGTGGAACGGGTGCAGAAATACCAGGCCTTGCTGAAGGAGCTAATCCGCAACAAGGCCCGCAACCGGCAGAACTGCGCGCTGCTGGAGCAGGCCTATGCTGTGGTGTCTGCCCTGCCTCAGCGCGCCGAGAACAAGCTGCACGTTTCTCTCATGGAGAACTACCCAGGAACCCTGGAGGCCCTGGGAGAACCTATCCGCCAGGGCCACTTCATAGTGTGGGAGGGGGCCCCAGGGGCCCGGATGCCTTGGAAGGGCCACAACCGGCACGTCTTCCTCTTCCGAAACTACCTGGTGATCTGCAAGCCCCGAAGAGACTCTCGAACCGACACCTTCAGCTATGTGTTCCGGAACATGATGAAGCTGAGTAGCATCGACCTGAATGACCAAGTAGAGGGGGATGACCGCGCCTTTGAGGTGTGGCACGAACGGGAAGACTCTGTGCGCAAGTACCTGCTGCAGGCACGGACCGTCATCATCAAAAACTCCTGGGTGAAGGAGATCTGTGGCATCCAGCAGCGCCTGGCCCAGCCTGTGTGGCGTCCCCCTGAGTTTGAAGAGGAACTAGCGGACTGCACAGCCGAGCTGGGTGAAACGGTCAAGCTAGCATGCCGAGTGACGGGCACACCTAAGCCTATTGTCAGCTGGTACAAAGATGGGAAGCCAGTGGAGGTGGACCCACACCACATCCTCATTGAAGACCCTGACGGCTCCTGCACCCTCATCTTGGACAACCTTACTGGCATAGACTCTGGCCAGTACATGTGTTTTGCGGCCAGTGCTGCTGGCAACGCCAGCACCTTGGGGAAGATTCTAGTACAAGTGCCCCCACGGTTTGTGAACAAGGTCCGGGCCACACCCTTTGTGGAGGGAGAGGATGCACAGATCACCTGCACAGTGGAAGGAGCCCCGTACCCTCAGATCAGGTGGTACAAGGATGGTACCCTGCTAGCCCCGGGCAACAGGTACCGGATGTTGAATGAACCCCGCAGTGGTGTGCTGGTGCTGGTGATCCAGGCGGCCAGCAAGGAGGACCTGGGACACTATGAATGTGAGTTGGTGAACCGGTTGGGCTCAACACGTGGTGGTGGAGAACTATACATGCAGAGCCCTGCACTGCGTGCCCGGGACCAGCACCACCGGGAACAGATTGTGGCTGCCGTAGAAGTCACTGAGCAGGAGACAAAAGTCCCCAAGAAAACCGTCATCATAGAGGAGACCATCACCACCGTAGTGAAGAGTCCGCGTGGCCGACGACAGTCCCCCGGCAAGTCTCCCTCTCGTTCACCTTCCCGCCGCTCTGCCAGTCCACGGAGACCAGGGCTGTTGGCCCCCGAGAGGCTGTATCCACCAGGGACCAGCCCATCCCGGAGGCTTGAAGTAGAACAAGGCAGGAAAGCCCCGGTGCCTGCATTGTATGTGACAGAAGCAGAGGTCCACGCTCCAGCCTCGCAGTCCCAGCCCAAGTGGTTAGAGGTGGAGGAAACCATTGAAGTCCGGGTGAAGAAGACGGGGTCACGGGGTGCATCTCCTGTCCGAGAGATGACCAGCCGTGGAGAAGGAATCCTCTTCACACTGCCTGGTGGGATCCCTGGTAGGGACCCAAATGCCAACAACTCCAACAACAAGAGTGTGTACCAGGAAGCCCGGACCTGGGGTCCCGCTGTGGTCCATGTTGGTGAACCTTTCATCTTCCAAGTGGACTCGGTGGGCAATGTGGACTGGGTAGCTGCTAGCCCTGAGCCAGAGCAGGTCAGAGCCTCCCAGAAGGAAGAGAACACTGAAAGGCAGGAAGGATGCAGTGATGGAGACGAGAATACCTTCCTGATGGAAGAACCACAGGACACGGATAGCCTTCAGGGCCGTGACCCCAAAATCCTCACACACAATGGCCGGGTGCTGACATTGGCTGACCTGGAAGATTATGTGCCCCAGGAAGGAGAGACTTTTGGCTGTGGGGACTCCACCCCCAGCACCCCTGATGAACCTCCCTGTGAAGTCTCCGTGCTCCAGAGAGAGATCAGCGAGCCCACGGTGGGGCAACCTGTCCTGCTCAATGTGGGGCGTCCTCCAGGCACCGGTGCCACTCCCAGCTTCTTCAGGCCAGGTTCCCAGGTCCACAGCCCTGAGAGTGTCTCCTTCCTTTTGCGTGAGGCCTGGTCAGGTCCAGTGAGTGCCGCCCCTTGGACAAGCTCCTTCCATACCCATGTCCAGAGTTCTGTAGATGGTAGCCATGGGAGCTTCAAGACAGAGGTCTCCACCCAGACAGTCAGCTTTGGGGCCGTAGGAGAGACAGTCACTCTTCATATTGACCCAGATGGTGGCGAGGCTCCAGGCCCCTCCCAGGGCTGA
| 载体名称: | pEGFP-N1 |
| 载体抗性: | Kanamycin (卡那霉素) |
| 筛选标记: | Neomycin (新霉素) |
| 筛选标记: | Neomycin / G418 |
| 5'测序引物: | CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3' |
pEGFP-N1 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences. Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site to further increase the translation efficiency in eukaryotic cells.
The MCS in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen. A neomycin-resistance cassette (Neor ), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pEGFP-N1 backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
让基因研究更简单!
您值得信赖的一站式基因研究合作伙伴!