pET-28a(+)-AFF1 小鼠AFF1原核表达载体
ATGGAAACGAGGATTCTCCCACGAGAGAGTGGTGGAGCCTGCTTGTACAATGAAGATAGAAACCTGCTTCGAATCAGAGAGAAGGAAAGACGCAACCAAGAAGCTCACCAGGAGAAGGAGGCATTTCCCGAGAAGGCTCCCCTGTTTCCAGAGCCTTACAAGACTGCAAAAGGCGATGAGCTATCAAGTCGGATCCAGACCATGCTGGGTGACTATGAGGAGATGAAGGAGTTCCTGAGCAGCAAGTCCCACCCCCACCGCCTGGATGGCTCTGAAGACAGGCCCGGGAAGCCCAGATATCCCTTAGGTCATGACAGGGGGAACGGGGCTGCATCCAGCTCCCTCCGCACACATGTCTACCACCAGCCTATCCACACTTCTGCTCCCGGATCACGTCCTGTCGGTAACATTAGCCACAGTCCAAAGATGGCACAGCCAAGGATGGAGCCAAGTCTCCACACCAAAATCTATGATGGCCCACGTCTGACTCAAGACCACCTCAGTCAGGGACATTGTTCCAGAAAGTGTGACCGAAGAGCTGAAGGAGACTCTGCTCCCGAGAGGAAGCTCTCGCCCTTGATCTCCTCTTTGCCATCCCCGGTGCCCCCTCTATCACCTGTACATTCCAGGCTGCAGGGAACCAGCAAGGCTCACAGCAGTGGCGTTAGCAGTAAAAGCTGCTGTGTGGCCAAGTCTTCCAAGGACTTGGTGGCGAAGGCCCAAGATAAAGAGACTCCTCATGACGGTTTGGTGGCAGTTACCAGCCTTGGATCAGCCCCTCCTCAGCCACCTTGCCAGACATTTCCACCCCCTCCTCTCCCCTCAAAAAGTGCTGCAATGCAGCAGAAGCCCACGGCATATGTCCGTCCCATGGACGGTCAGGATCAGGCTCCCAGTGAGTCCCCCGAGCTGAAGCTGCCACTGGAGGACTATGGTCAGCAAAGCTTTGAGAAACCAGACCTTAAAGTGCCTGCCAAAGCCAAGCTCACCAGACTAAGGATGCCCTCTCAGTCGGTGGAGCAGCCGTACTCCAATGAAGTCCATTGCGTTGAAGAGATTCTGAAGGAAATGACCCACTCGTGGCCACCGCCTCTGACAGCCATACACACACCCAGTACAGCCGAGCCGTCCAGATTTCCTTTCCCCACAAAGGACCCTCTGCACGTCAGTCCTGCAACCCAGAGCCAAAAACAATATGACACGCCTTCAAAAACCCACCCCAATCCCCAGCAAGGAACCTCCATGCTGGAGGACGACCTGCAGCTCAGCGACAGTGAAGACAGCGACACGGAGCAAGCCACAGAGAAGCCTCCATCCCCACCTGCACCTCCCAGCGCTCCGCAGACACTTCCCGAGCCCGTGGCCTCAGCACATTCCAGTAGTGGGGAGTCAGAGAGCAGCGAGTCAGACAGTTCCTCAGACTCTGAGAGTGAGAGCAGCTCCAGCGACAGCGAGGAGGAGGAAGAGAACGAGCCCCTGGAGACCCGGGCTCCCGAGCCCGAGCCTCCGACGACAAACAAATGGCAGCTGGACAACTGGTTGACCAAAGTCAACCAACCCTCAGTGCCCCTGGATGGCCGGGGGAGCACAGAGTCTCCACAATGGCGCCAGGAAAGTAAGGGTGTCGCTGAGGGCAGCAGTGACCAGCAGCATCCTGATTCCAAAGACCCTCTCCCAAAGAGCTCCAGCAAGACTCTCCGGGGTCCCTCTGAGGGGCCCCAGCCTGGGAAGAGGGGCTGTCCGAAATCCCCCGCTCAACAGGAACCCCCACCTCGGCAAACCGTGGGCAGCAAACAACCCAGGAAACCCGCCAAGGGCTCCGGCCAGGCAGAGCCCCAGGCCAGCTCGCAGGCAGAGAGTGAAGTAGGACCCCTTCCCTACGGCTCAAAGGAGCAGACTTCCAAAGACAGACCCAAGGTGAAGACGAAAGGCAGGCCGCGTGCCGTGGGCAGTCGGGAGCCTAAGCCAGAGGTCCCTGCGCCCACGCCTCAGGCAGCCGTGCCCAGACCGAAGCCGCCTGTGCCCACCCCCAGTGAGAAGAGGAAGCACAAGAGCTCCACGGCCCCCTCCAAGGCGCCCTCAGCCCCCCAGCCTCCGAAGGACAGTGCTGGGGACAGGAACCCAGAACACTCTGCTCTTGTCTCCTTGACTCAGAGCCAGGGTCCATCCCACAGCAGCAGGGGCAGCAGTGGCAGCGTCAGGACTAGTGGCTGCCGACAGGCTGTGATTGCCCAAGGGGACGGCTGTAAAGACAAGCTCCTGTTGCCTTTGAGAGACACCAAATTGCTCTCGCCGCTCAGGGACTCCCCACCACCGACAAGCTTGGTGGTGAAGATAACCCTAGACCTCCTCACGAGGATTCCCCAGCCTCTGGGGAAGGGGAGCCGCCCCAGGAAAGCAGAAGACAAGCAGCTGTCAGCAGGGAAGAAGCAGGACTCGGAGACCAAGAGCTGTGACAGCTCCAGCAGAGTGACCAAGAAGAGAAAGGGTGACCCAGAAAAAGAGCACAGTAACAAGAGACACAAACTGGATAAGTCGCAGACTGCGTCATCTTCATCTTCCCATACAGAATCTTCCAGAACAAAAGCGCCCAGGTCCTCCTCAGAGAACTCAAGAAAGGAAATGCTGCCTCCAGCATCGGCGTCCTCAGTCTCCTCTTCCTCATCCTCCCAGAAGCCAAGCAGACCTGCACAGAAGAGGCCAAGGCCAGACGAAGACACGTGTAGCCAGGAACCCCCCAGAAGTGCCAGCAGCACAAAGAGCAGCTCTACAGACCCTCCCGCTCCCAAGCACAGAAAAGTACAGGCCAGGGGCTCTGAACACAAGGGATCTTCTGGGGATGCTGCGAATGCTGCAAATCCTTTTCCAGTGCCTTCTTTGCCGAATGGTAACGCTAAACCAGGGAAGCCACAGGTGAAGTCTGACAGACAACAAGCTGACTTTCACATGAAGGAGGCTAAAAAGCTGAAGTGCAAAGCAGAGACAATGGTAGACAAGGCTGGAAAAGCCTTCAAGTATTTGGAAGCCGTCCTCTCTTTTATCGAGTGTGGAATGGCCTCAGAGTCAGAAAGCTCAGCCAAGTCGGCGTACGCTGTGTACTCAGAAACCATAGACCTCATTAGGTATGTGATGTCACTAAAATGCTTCTCAGATAACACGATGCCAGCACAAGAGAAGATATTTGCTGTTTTATGCTTGCGTTGCCAGTCCCTTTTGAACATGGCGATGTTTCGATGTAAGAAAGACACAGTGATGAAGTATTCTCGTACTCTCAGTGAACACTTCAAGAGTACTTCCAAAGTGGCCCAGGCACCTTCTCCATGCACGGCAAGAAGCACAGGCGTTCCGTCCCCCCTCTCCCCAATGCCTTCTCCTGCCAGCTCCGTGGGGTCCCAATCAAGTGCTGGCAGCAGTATGGGGAGCGTCGGGGTGACGGCCACCGTCAGTACTCCAGTCTCCATCCAGAACATGACCTCCTCCTATGTCACCATCACATCCCATGTCCTTACGGCCTTTAGCCTTTGGGAACAGGCCGAGGCCCTCACAAGGAAGAACAAAGAATTCTTCGCTCAGCTCAGCACAAAAGTGCGTGTGTTGGCCCTCAACAGCAGCCTGGTGGACCTGGTGCACTACACAAGACAGGGCCTGCAGCGGCTAAAACAGTCACCCAAAGGCCCCTAA
| 载体名称: | pET-28a(+) |
| 载体抗性: | Kanamycin (卡那霉素) |
| 表达类型: | 高表达 |
| 5'测序引物: | T7: 5'-TAATACGACTCACTATAGGG-3' |
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
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