pMD18-T-PCM1 小鼠PCM1基因cDNA克隆
ATGGCCACAGGAGGAGGTCCTTTTGAAGAAGTCATGCATGATCAGGACTTACCAAACTGGAGCAATGACAGTGTGGATGACCGACTCAACAATATGGAGTGGGGTGGCCAACAGAAGAAGGCCAATAGATCATCAGAAAAGAATAAGAAAAAGTTTGGTGTAGCAAGTGATAAGAGGGTAACTAACGCTATTTCTCCGGAGTCATCGCCAGGAGTTGGAAGGCGAAGAACAAAGATTCCACATACGTTTCCACACAGCAGATATATGACTCAGATGTCTGTTCCAGAGCAGGCAGAACTAGAGAAACTTAAACAGCGGATAAACTTCAGTGATTTAGATCAGAGAAGTATTGGAAGTGATTCTCAAGGTAGAGCAACAGCGGCTAACAACAAACGTCAGCTTAGTGAAAACCGAAAGCCCTTCAACTTTTTGCCTATGCAGATTAATACTAACAAGAGCAAGGATGCTACTGCAAGTCTTCCAAAGAGAGAGATGACAACGTCAGCACAGTGCAAAGAGTTGTTTGCTTCTGCTCTAAGTAATGACCTTTTGCAAAACTGTCAAGTCTCTGAAGAAGATGGGAGAGGGGAGCCTGCAATGGAGAGCAGCCAGATTGTAAGCAGGCTTGTTCAAATCCGTGACTATATTACTAAAGCTAGTTCTATGCGAGAAGATCTTGTAGAGAAAAATGAAAGATCTGCCAATGTTGAGCGTCTTACTCATCTAATAGAGCATCTTAAAGAGCAAGAAAAGTCATATATGAAATTTCTCCAGAAAATCCTTGCCAGAGATCCTCAGCAGGAGCCTATGGAAGAGACAGAAAATTTGAAGAAACAACATGATTTATTAAAAAGAATGTTACAACAGCAGGAGCAGCTGCGAGCTCTGCAGGGCCGACAGGCTGCTCTTCTAGCTTTGCAGCATAAAGCAGAGCAAGCTATAGCTGTGATGGATGACTCTGTTGTTACAGAAACTACAGGAAGCTTATCTGGAGTCAGCATCACATCTGAACTAAATGAAGAACTGAATGATTTAATTCAGCGTTTCCATAATCAGCTTCGTGATTCTCAGCCTCCAGCTGTTCCAGACAACAGAAGACAGGCAGAAAGTCTTTCATTAACTAGAGAGATTTCTCAGAGCAGAAATCCCTCAGTTTCTGAACATTTACCTGATGAGAAAGTACAGCTTTTTAGCAAAATGAGAGTACTACAGGAAAAGAAACAGAAAATGGACAAATTACTTGGAGAACTTCATAACCTTCGAGATCAGCATCTGAACAACTCATCATTTGTGCCTTCAACTTCTCTCCAAAGAAGTGGGGATAAGAGAAGTTCAACTGTAGCTCTCTCTGCTCCTGTGGGCTTTGCATCAGCTGTCAATGGAGAGGCCAATAGTCTTATCTCATCTGTTCCCTGTCCTGCCACCTCTCTAGTTTCTCAGAATGAGAGTGAAAATGAAGGTCATCTAAATCCAGCTGAAAAACTACAGAAGTTAAATGAAGTTCAAAAGAGATTGAATGAACTGAGAGAATTAGTTCATTATTATGAACAAACATCAGATATGATGACAGATGCTGTAAATGAAAATACAAAAGATGAAGAAACTGAAGAGTCAGAATATGATTCTGAACATGAAAATTCTGAACCTGTTACTAACATTAGAAATCCACAGGTAGCTTCTACCTGGAATGAAGTGAATACTAACAGTAATACACAGTGTGGTTCTAACAATAGAGATGGGAGACCAGTTAATTCTAACTGTGAAATTAATAACAGATCTGCTGCCAACATCAGAGCTCTAAACATGCCTCCATTAGACTGTCGATACAATAGAGAAGGAGAGCAGAGGTTGCATGTAGCACATGGAGAAGATGAGGAAGAAGAAGTAGAAGAGGAAGGAGTCAGTGGGGCTTCATTATCTAGTCGTAGGAGCAGTCTGGTCGATGAAGCACCAGAAGATGAAGAATTTGAACAGAAGATTAGTAGACTCATGGCTGCAAAAGAGAAACTTAAACAGCTCCAAGATCTTGTTGCTATGGTGCAGGATGATGATGCAACTCAAGTGGTTGTCCCTGCTGCATCAAATTTGGATGATTTCTATGCAGCAGAAGAAGATATCAAACAAAATTCCAATAATGCAAGAGAAAATTCTAATAAAATAGATACTGGAGTAAATGAAAAAACAAGAGAGAAATTTTATGAGGCTAAACTACAGCAGCAACAGAGAGAGCTTAAACAATTGCAGGAGGAAAGAAAGAAATTGATTGAAATTCAAGAGAAAATTCAAGCAGTGCAAAAGGCATGCCCTGACCTTCAGCTCTCAGCCACTAGTATAAGTAGTGGTCCCACAAAAAAATATCTACCAGCTATTACTTCAACTCCAACTGTTAATGAAAATGACAGCAGTACAAGCAAGTGTGTTATTGATCCTGAAGATTCTTCAGTGGTGGATAATGAGTTGTGGTCAGACATGAGAAGACATGAAATGTTAAGGGAAGAGCTACGGCAGAGAAGGAAGCAACTTGAAGCTCTAATGGCTGAACACCAGAGGAGGCAAGGTCTAGCTGAGACCTCATCTCCAGTGGCTATATCATTGAGAAGTGATGGATCTGAGAACCTGTGCACTCCTCAGCAAAGTAGAACAGAAAAAACAATGGCAACTTGGGGAGGTTCTACTCAGTGTGCATTAGATGAAGAAGGAGATGAGGATGGCTATCTTTCAGAAGGAATTGTTCGCACAGATGAAGAAGAGGAGGAGGAGCAGGATGCCAGTTCCAATGATAACTTCCCTATATACCCTCCTAGCATGAATCAAAATTCTTACAATGTAAAGGAAACCAAAACCAGGTGGAAGAGCAATCGCCCTGTTTCAGCAGATGGAAATTACCGTCCTTTAGCCAAGACAAGGCAGCAGAATATCAGCATGCAACGACAAGAAAACCTTCGATGGGTGTCAGAGCTCTCTTACATAGAAGAGAAGGAGCAGTGGCAAGAACAGATTAATCAGCTAAAGAAACAACTCGATTTCAGTGTCAATATTTGTCAGACTCTGATGCAAGACCAACAGACTTTATCTTGTTTGCTGCAGACTCTTCTAACAGGTCCGTACAGTGTTCTGCCTAGTAATGTGGCGTCCCCTCAAGTGCACCTTATCATGCATCAGCTGAACCAATGCTACACACAGCTAACATGGCAGCAGAACAATGTCCAGAGGTTAAAACAAATGCTAACTGAACTTATGCGCCAACAAAATCAGCACCCAGAAAAGCCTAGAAGCAAAGAAAGAGGCAGTAGTGCGTCACACCCTTCTTCTCCCAATTTATTTTGCCCTTTCAGCTTTCCAACACAGCCTGTAAATCTGTTTAACCTGCCTGGATTTACTAACTTTCCATCATTTGCACCAGGAATGAATTTCAGCCCTTTATTTCCTTCTAATTTTGGAGATTTTTCTCAGAATGTTTCTACACCTACTGAACAGCAGCAGCCATTAGCTCAAAATCCTTCAGGGAAAACAGAGTATATGGCTTTTCCAAAACCCTTTGAAAGCAGTTCTTCTCTTGGAGCAGAAAAGCAAAGGAATCAGAAACAGCCCGAAGAGGAAGCTGAAAACACTAAGACACCATGGTTATATGATCAAGAAGGTGGAGTAGAAAAACCATTTTTCAAGACTGGATTTACAGAGTCTGTAGAGAAAGCTACAAATAGTAACCGCAAAAATCAACCAGATACAAGCAGGAGAAGACGTCAGTTTGATGAAGAATCCTTGGAAAGCTTTAGCAGTATGCCTGATCCTATAGACCCAACAACAGTAACTAAAACATTTAAAACAAGAAAAGCATCTGCCCAGGCCAGCCTGGCCTCTAAGGACAAAACTCCCAAATCAAAGAGTAAGAAGAGGAATTCTACTCAGCTGAAAAGTAGAGTTAAAAATATTGGGTATGAAAGTGCCAGTGTGTCTAGCACATGTGAACCTTGCAAAAATAGGAACAGACATTCAGCCCAGACTGAAGAGCCTGTTCAAGCAAAATTATTCAGCAGAAAGAATCATGAACAGCTGGAAAAAATAATAAAATATAGTAGGTCTGCAGAAATATCTTCAGAGACTGGAAGTGATTTTTCCATGTTTGAAGCTTTACGGGATACTATTTATTCCGAAGTGGCTACATTAATTTCTCAAAATGAGTCTCGTCCACATTTTCTTATTGAACTTTTCCATGAGCTGCAGCTACTTAATACAGACTACTTGAGACAGAGAGCTTTATATGCATTGCAGGATATCGTATCCAGACACATTTCTGAAAGTGATGAAAGAGAAGGAGAAAATGTAAAGCCTGTGAACTCTGGCACTTGGGTAGCATCCAATTCAGAGCTTACTCCGAGTGAGAGCCTTGTTACTACTGACGATGAAACATTTGAGAAGAACTTTGAAAGAGAAACACATAAAGTAAGTGAACAGAATGATGCTGACAACGTGAGTGTCATGTCCGTATCCTCCAACTTTGAGCCATTTGCAACAGACGACTTAGGTAACACTGTGATTCACTTGGATCAAGCATTAGCCAGAATGAGAGAGTATGAGCGCATGAAGACCGAGACAGAAAGTCACTCAAATATGAGATGCACTTGCAGGGTTATTGAGGATGAAGATGGTGCTGCCGCCGCTGCCACAGTCAGTAATTCAGAAGAAACTCCCATTATTGAGAATCACAATTCACCACAACCTATAAGTGATGTTTCTGCCGTCCCCTGTCCTAGAATTGATACTCAGCAACTGGACCGCCAAATTAAAGCAATTATGAAAGAAGTCATTCCTTTTTTGAAGGAGCACATGGATGAAGTGTGTTCGTCTCAGCTTCTCACTTCAGTAAGACGTATGGTCTTGACCCTTACCCAGCAGAATGATGAGAGCAAAGAGTTTGTGAAGTTCTTTCATAAACAACTAGGAAGTATATTGCAGGATTCACTAGCAAAATTTGCAGGCAGAAAGCTGAAAGACTGTGGAGAAGATCTTCTTGTGGAGATCTCTGAAGTGTTGTTTAATGAATTGGCTTTCTTTAAGCTCATGCAAGACTTGGATAATAATAGCATAGCTGTTAAACAGAGGTGCAAGAGGAAAATTGAAGCAGCTGGAGTGAGGCAGTCTTATGCAAAAGAGGCCAAAAGGATTCTTGAAGGAGATCATGGCTCACCTGCTGGAGAGATTGATGATGAAGACAAAGACAAGGATGAGACTGAAACTGTTAAGCAGACTCAGACTTCTGAGGTATATGATGCTAAAGGTCCCAAAAATGTAAGATCTGATGTTTCTGATCAAGAAGAAGATGAAGAAAGTGAACGGTGTCCAGTGTCTATTAATCTGTCTAAAGCTGAAAGTCAGGCTTTAACTAATTATGGAAGTGGAGAAGATGAGAATGAGGATGAAGAAATGGAAGACTTTGAAGAGAGTCCTGTGGACATCCAGACTTCACTTCAGGCTAACACTGAAACTACAGAAGAAAATGAACATGACAGTCAGATTCTGCAACATGATCTTGAAAAAACACCAGAAAGTACAAATGTACCATCAGACCAAGAACCAACTAGTAAGAATGATCAAGACAGCTCTCCTGTGAAACCTTGTTATCTCAATATCTTGGAAAATGAGCAACAGTTAAATAGTGCTACCCATAAGGACTCGCTTACTACTACTGATTCATCTAAACAGCCTGAGCCTCTGCCATTACCCTTGGCTGCAAGTGAAACCTTAGTGCCTAGAGTCAAAGAAGTGAAGTCTGCTCAGGAAACGCCTGAGAGCTCTCTGGCTGGAAGCCCTGATACTGAATCTCCAGTGTTAGTGAATGACTATGAAGCAGAGTCTGGTAACATAAGTCAAAAGTCGGATGAAGAAGACTTTGTGAAAGTTGAGGATTTGCCGCTCAAACTGACAGTCTATTCAGAGGAAGAGCTAAGGAAGAAAATGATAGAAGAAGAACAGAAAAACCATTTATCTGGTGAAATATGTGAAATGCAGACAGAAGAATTAGCTGGAAATTCTCAGATACTGAAAGAACCTGAAACAGTGGGAGCCCAGAGTATATGA
pMD18-T载体是由pUC18载体改建而成的。在pUC18载体的多克隆位点处的XbaI和SalI识别位点之间插入了EcoRV识别位点，用EcoRV进行酶切反应后，在两侧的3’端添加“T”而成。这种构建方式使得pMD18-T载体非常适合于克隆PCR产物。
pMD18-T载体在分子生物学实验中得到了广泛的应用，特别是在基因克隆、表达、测序和分析等方面。例如，研究人员可以利用pMD18-T载体进行TA克隆，将PCR产物插入载体中，然后将其转化到大肠杆菌等宿主细胞中进行复制和表达。此外，pMD18-T载体还可以用于构建表达载体，用于表达目的蛋白的研究。
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
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