M50 Super 8x TOPFlash信号通路报告载体

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载体订购信息

产品编号
GS-8105
产品名称
M50 Super 8x TOPFlash 信号通路报告载体
市场价格
￥ 1000
沟通洽谈

载体基本信息

载体名称:
M50 Super 8x TOPFlash
载体类型:
信号通路报告载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin
筛选标记:
/
载体大小:
4985 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
Luc

载体图谱展示

载体图谱

载体说明

This is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites.

This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 7 TCF/LEF binding sites were cloned into the Mlu1 site of this vector (7 copies of: AGATCAAAGGgggta, with TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the TCF/LEF site).

Note: This plasmid was published as M50 Super 8x TOPFlash, but the plasmid actually contains 7 TCF/LEF sites.

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