pET-47b(+)原核表达载体

载体基本信息

载体说明

The pET-47b(+) plasmid features the ability to express fusion proteins with an N-terminal His?Tag? coding sequence that is cleavable with the human rhinovirus (HRV) 3C protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S?Tag? coding sequence is compatible with purification, detection, and quantification.