pFUSE-hIgG1e1-Fc1Fuse融合蛋白表达载体

载体基本信息

载体说明

pFUSE-Fc is a family of plasmid developed to facilitate the construction of Fc-fusion proteins by fusing the effector region of a protein to the Fc region of an immunoglobulin G (IgG). pFUSE-Fc plasmids yield high levels of Fc-fusion proteins. The level of expression is usually in the μg/mL range. They can be transfected in a variety of mammalian cells. pFUSE-Fc2 (IL2ss) plasmids allow the secretion of Fc-Fusion proteins. They contain the IL2 signal sequence (IL2ss) for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted. As Fc-Fusion proteins are secreted, they can be easily detected in the supernatant of pFUSE-Fc-transfected cells by SDS-PAGE. Furthermore, functional domains can be identified by immunoblotting and ligand blotting. Fc-Fusion proteins can be easily purified by single-step protein A or protein G affinity chromatography.