载体说明
Features of the pGIPZ? lentiviral vector (Figure 2-3, Table 1) that make it a versatile tool for RNAi studies include:
- Ability to perform transfections or transductions using the replication incompetent lentivirus (Shimada, et al. 1995)
- TurboGFP and shRNAmir are part of a bicistronic transcript allowing the visual marking of shRNAmir expressing cells
- Amenable to in vitro and in vivo applications
- Puromycin drug resistance marker for selecting stable cell lines
- Molecular barcodes enable multiplexed screening in pools