pAc5.1/V5-His B果蝇表达载体

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载体订购信息

产品编号
GS-1088
产品名称
pAc5.1/V5-His B 果蝇表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pAc5.1/V5-His B
载体类型:
果蝇表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
5379 bp 点击查看完整序列
启动子:
P-AC5 (Actin 5C)
测序引物5’:
AC5:?5'd[ACACAAAGCCGCTCCATCAG]3'
测序引物3’:
/
报告基因:
/

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载体说明

The DES?-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the?Drosophila?actin 5C gene. The pAc5.1/V5-His vector also offers the following features:

  • Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
  • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.

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