pACT2 AD酵母表达载体

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载体订购信息

产品编号
GS-1646
产品名称
pACT2 AD 酵母表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pACT2 AD
载体类型:
酵母表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
8117 bp 点击查看完整序列
启动子:
ADH1
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pACT2 generates a fusion of the GAL4 AD (amino acids 768–881), an HA epitope tag, and a protein of interest (or protein encoded by a cDNA in a fusion library) cloned into the MCS in the correct orientation and reading frame. pACT2, which is derived from pACT (1), contains a unique EcoR I site in the MCS. The hybrid protein is expressed at high levels in yeast host cells from the constitutive ADH1 promoter (P); transcription is terminated at the ADH1 transcription termination signal (T). The protein is targeted to the yeast nucleus by the nuclear localization sequence from SV40 T-antigen which has been cloned into the 5’ end of the GAL4 AD sequence (2). pACT2 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae and carries the bla gene, which confers ampicillin resistance in E. coli. pACT2 also contains the LEU2 nutritional gene that allows yeast auxotrophs to grow on limiting synthetic media. Transformants with AD/library plasmids can be selected by complementation by the LEU2 gene by using an E. coli strain that carries a leuB mutation (e.g., HB101).

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