pcDNA3.1(-)哺乳动物表达载体

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载体订购信息

产品编号
GS-1010
产品名称
pcDNA3.1(-) 哺乳动物表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pcDNA3.1(-)
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin/G418
载体大小:
5427 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
测序引物3’:
pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
报告基因:
/

载体图谱展示

载体图谱

载体说明

pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: ? Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells ? Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning ? Neomycin resistance gene for selection of stable cell lines ? Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.

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