载体订购信息

产品编号
GS-1031
产品名称
pCEP4 哺乳动物表达载体
市场价格
¥ 1000
沟通洽谈

载体基本信息

载体名称:
pCEP4
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Hygromycin (潮霉素)
载体大小:
10186 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
测序引物3’:
EBV-R:5'-GTGGTTTGTCCAAACTCATC-3'
报告基因:
/

载体图谱展示

载体图谱

载体说明

pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.

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