pCOLAduet-1原核表达载体

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载体订购信息

产品编号
GS-1240
产品名称
pCOLAduet-1 原核表达载体
市场价格
￥ 1500
沟通洽谈

载体基本信息

载体名称:
pCOLAduet-1
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Kanamycin (卡那霉素)
筛选标记:
/
载体大小:
3719 bp 点击查看完整序列
启动子:
/
测序引物5’:
T7: 5'-TAATACGACTCACTATAGGG-3';
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His?Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S?Tag? peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.

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