pGBKT7酵母表达载体

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载体订购信息

产品编号
GS-1638
产品名称
pGBKT7 酵母表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pGBKT7
载体类型:
酵母表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Kanamycin (卡那霉素)
筛选标记:
/
载体大小:
7303 bp 点击查看完整序列
启动子:
ADH1
测序引物5’:
T7 Forward
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pGBKT7 vector expresses proteins fused to amino acids 1–147 of the GAL4 DNA binding domain (DNA-BD). In yeast, fusion proteins are expressed at high levels from the constitutive ADH1 promoter (PADH1); transcription is terminated by the T7 and ADH1 transcription termination signals (TT7 & ADH1). pGBKT7 also contains the T7 promoter, a c-Myc epitope tag, and a MCS. pGBKT7 replicates autonomously in both E. coli and S. cerevisiae from the pUC and 2 μ ori, respectively. The vector carries the Kanr for selection in E. coli and the TRP1 nutritional marker for selection in yeast. Yeast strains containing pGBKT7 exhibit a higher transformation efficiency than strains carrying other DNA-BD domain vectors (1).

载体应用

pGBKT7 is the DNA-BD Vector included with Clontech's Matchmaker? Systems. The MCS of pGBKT7 contains unique restriction sites in frame with the 3' end of the GAL4 DNA-BD for constructing fusion proteins with a bait protein. The bait protein is also expressed as a fusion to a c-Myc epitope tag. c-Myc tagged proteins can be identified with antibodies raised to this common epitope, eliminating the need to generate specific antibodies to new proteins. The T7 promoter is used for in vitro transcription and translation of the epitope tagged fusion protein. Note that the DNA-BD is not expressed during the in vitro transcription and translation reactions.

The MCS in pGBKT7 is compatible with those in pMyc-CMV and pHA-CMV, Clontech's epitope tagged mammalian expression vector set (Cat. No. 631604). As a result, the target gene can be shuttled into these vectors in order to confirm protein interactions in vivo.

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