pGene/v5-His B哺乳动物表达载体

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载体订购信息

产品编号
GS-1042
产品名称
pGene/v5-His B 哺乳动物表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pGene/v5-His B
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Zeocin (博来霉素)
载体大小:
4654 bp 点击查看完整序列
启动子:
Gal4-E1b
测序引物5’:
pGene Fwd:?5'd[CTGCTATTCTGCTCAACCT]3'
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The GeneSwitch? System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.To activate transcription from the GAL4-E1b promoter, the GeneSwitch? regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch? protein has three functional domains:

  • Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
  • Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone

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