pLNCX2逆转录病毒载体

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载体订购信息

产品编号
GS-1532
产品名称
pLNCX2 逆转录病毒载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pLNCX2
载体类型:
逆转录病毒载体
克隆方法:
/
拷贝类型:
低
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (新霉素)
载体大小:
6132 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
pLNCX-F: AGCTGGTTTAGTGAACCGTCAGATC
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pLNCX2 contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1– 3). Upon transfection into a packaging cell line, pLNCX2 can transiently express, or integrate and stably express, a transcript containing Y+ (the extended viral packaging signal) a selectable marker, and the gene of interest. The 5' viral LTR in this vector contains viral promoter/enhancer sequences that control expression of the neomycin resistance (Neor) gene for antibiotic selection in eukaryotic cells. A gene of interest can be cloned into the multiple cloning site immediately downstream of the human cytomegalovirus (CMV) immediate early promoter (PCMV). pLNCX2 also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.

载体应用

pLNCX2 can be transfected into a packaging cell line such as the RetroPackTM PT67 Cell Line (#K1060-D). Once in the cell, RNA from the vector is packaged into infectious, replicationincompetent retroviral particles. pLNCX2 does not contain the structural genes (gag, pol, and env) necessary for particle formation and replication; these genes are stably integrated into PT67 (4–7). Subsequent introduction of pLNCX2, containing Y+, transcription and processing elements, and the gene of interest produces high-titer, replication-incompetent infectious virus. These retroviral particles can infect target cells and transmit the gene of interest (which is cloned between the viral LTR sequences), but cannot replicate within these cells since the cells lack the viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chances of producing replication-competent virus due to recombination events during cell proliferation.

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