pMT/BiP/V5-His A昆虫细胞表达载体

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载体订购信息

产品编号
GS-1177
产品名称
pMT/BiP/V5-His A 昆虫细胞表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pMT/BiP/V5-His A
载体类型:
昆虫细胞表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
3642 bp 点击查看完整序列
启动子:
MT
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The DES?-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:

  • Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
  • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
  • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.

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