pTet-On-Advanced四环素调控系统

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载体订购信息

产品编号
GS-1603
产品名称
pTet-On-Advanced 四环素调控系统
市场价格
￥ 1000
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载体基本信息

载体名称:
pTet-On-Advanced
载体类型:
四环素调控系统
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin（新霉素）
载体大小:
7139 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

pTet-On-Advanced expresses an improved version of the reverse Tet (tetracycline)-controlled transactivator protein (rtTA), called rtTA-Advanced (1–4). It is more sensitive to doxycycline (Dox) and yields lower background expression than the original rtTA used in the Tet-On? System (2). The rtTA-Advanced protein is a fusion of amino acids 1–207 of a mutant Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains from the VP16 protein of herpes simplex virus. It is fully synthetic, lacks cryptic splice sites, and is codon-optimized for stable expression in mammalian cells.

载体应用

The pTet-On-Advanced Vector is used to develop stable Tet-On Advanced cell lines, which are hosts for a Dox-induced gene expression system. Once a vector containing a gene of interest under control of a Tet-responsive element (e.g., TRE-Tight or TRE2) is transfected into a Tet-On Advanced cell line, rtTA-Advanced binds to the TRE, and activates transcription of the gene of interest in the presence of Dox in a highly dose-dependent manner. Additional information on TRE-containing vectors and protocols describing the construction of Tet-On Advanced cell lines can be found in the Tet-On Advanced Inducible Gene Expression System User Manual (PT3898-1).

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