载体订购信息

产品编号
GS-1609
产品名称
pTK-Hyg 四环素调控系统
市场价格
¥ 1000
沟通洽谈

载体基本信息

载体名称:
pTK-Hyg
载体类型:
四环素调控系统
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Hyg
载体大小:
5066 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable cell lines using the Tet-On? or Tet-Off? Gene Expression Systems because the absence of an enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived plasmids upon cointegration into the host cell's genome.

载体应用

pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains a gene of interest using any standard transfection technique. Most mammalian cell lines require 200 μg/ml of Hygromycin to select for stably transformed cells.

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