pTRE2hyg四环素调控系统

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载体订购信息

产品编号
GS-1723
产品名称
pTRE2hyg 四环素调控系统
市场价格
￥ 1000
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载体基本信息

载体名称:
pTRE2hyg
载体类型:
四环素调控系统
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Hygromycin (潮霉素)
载体大小:
5325 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

pTRE2hyg is a response plasmid that expresses a gene of interest (Gene X) in CLONTECH's Tet-On? and Tet-Off? Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen?et al. (3; Tet-On). pTRE2hyg contains an MCS immediately downstream of the Tet-responsive?PhCMV*-1?promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively.?PhCMV*-1?contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). The TRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently,?PhCMV*-1?is silent in the absence of binding of TetR or rTetR to the?tetO?sequences. Note that the cloned insert must have an initiating ATG codon. In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE2hyg also contains the hygromycin resistance gene for direct selection of stable transformants. The parental vector pTRE2 was originally described as pUHD10-3 in reference 5.

The pTRE2hyg-Luc Control Vector, packaged with the pTRE2hyg Vector, contains an additional 1649 bp encoding firefly luciferase inserted into the MCS. This vector can be used as a reporter of induction efficiency using standard luciferase detection reagents. It is not intended as a cloning vector.

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