pVSV-G慢病毒载体

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载体订购信息

产品编号
GS-1470
产品名称
pVSV-G 慢病毒载体
市场价格
￥ 1200
沟通洽谈

载体基本信息

载体名称:
pVSV-G
载体类型:
慢病毒载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
6507 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pVSV-G expresses the G glycoprotein of the vesicular stomatitis virus (VSV-G) under the control of the CMV immediate-early promoter (1). VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus (MMLV)-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipid components of the target cell membrane and fosters the fusion of viral and cellular membranes (2). VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. pVSV-G Vector includes IVS, a synthetic intron known to enhance the stability of the mRNA (3), the Col E1 origin of replication and bacterial ampicillin rsistance (Ampr) gene for propagation and antibiotic selection in bacteria.

载体应用

As part of the Retro-X? Universal Retroviral Expression System (Cat. No. 631530), pVSV-G is cotransfected with a retroviral expression vector into the GP2-293 Packaging Cell Line (4) to produce infectious, replication-incompetent retrovirus. The genes encoding the viral gag and pol proteins are stably integrated into GP2-293. Because the VSV-G envelope protein causes toxicity by fusing cellular membranes, it must be expressed transiently from pVSV-G during packaging (5). Although the resulting virus can infect target cell lines and transmit a gene-of-interest, it cannot replicate because target cell lines lack the viral structural and polymerase/integrase genes. The separate introduction and integration of the viral genes into the packaging cell line and the use of minimal viral sequences in the vector minimize the chance of producing replication-competent virus due to recombination events.

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