pYD1酵母表达载体

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载体订购信息

产品编号
GS-1629
产品名称
pYD1 酵母表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pYD1
载体类型:
酵母表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
TRP1
载体大小:
5009 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
pYD1-F:?AGTAACGTTTGTCAGTAATTGC
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pYD1 is a 5.0 kb expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells. Features of this vector allow regulated expression, secretion, and detection of expressed proteins (see pages 15-16 for more information). The vector contains the following elements:

  • AGA2 gene from Saccharomyces cerevisiae. This gene encodes one of the subunits of the a-agglutinin receptor. Fusion of the gene of interest to AGA2 allows secretion and display of the protein of interest.
  • GAL1 promoter for regulated expression of the AGA2 gene fusion.
  • Xpress?epitope and V5 epitope for detection of the displayed protein.
  • Polyhistidine (6xHis) tag for detection and possible purification on metal chelating resin.
  • TRP1 gene for selection in Saccharomyces cerevisiae.
  • CEN6/ARS4 for stable, episomal replication in yeast.
  • Ampicillin resistance gene and the pUC origin for selection and replication in E. coli.

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