载体说明
pYD1 is a 5.0 kb expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells. Features of this vector allow regulated expression, secretion, and detection of expressed proteins (see pages 15-16 for more information). The vector contains the following elements:
- AGA2 gene from Saccharomyces cerevisiae. This gene encodes one of the subunits of the a-agglutinin receptor. Fusion of the gene of interest to AGA2 allows secretion and display of the protein of interest.
- GAL1 promoter for regulated expression of the AGA2 gene fusion.
- Xpress?epitope and V5 epitope for detection of the displayed protein.
- Polyhistidine (6xHis) tag for detection and possible purification on metal chelating resin.
- TRP1 gene for selection in Saccharomyces cerevisiae.
- CEN6/ARS4 for stable, episomal replication in yeast.
- Ampicillin resistance gene and the pUC origin for selection and replication in E. coli.
