pZsYellow原核表达载体

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载体订购信息

产品编号
GS-1828
产品名称
pZsYellow 原核表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pZsYellow
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
3331 bp 点击查看完整序列
启动子:
lac
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

pZsYellow is a pUC19-derived prokaryotic expression vector, which encodes a variant of wild-type Zoanthus sp. yellow fluorescent protein (ZsYellow) that has been engineered for brighter fluorescence. Wild-type ZsYellow cDNA was originally cloned from a Zoanthus species of coral, native to the Indian and Pacific oceans (1). A single amino acid substitution (Met-128 to Val) has been made to enhance the emission characteristics of ZsYellow (excitation maximum = 531 nm; emission maximum = 540 nm).

The ZsYellow gene was inserted in frame with the lacZ initiation codon from pUC19 so that ZsYellow is expressed from the lac promoter (Plac) in E. coli. The ZsYellow coding sequence is flanked by distinct multiple cloning sites (MCS) at the 5' and 3' ends so that the gene can be easily excised from pZsYellow and subcloned into other expression vectors. An upstream sequence—located just 5' to the ZsYellow gene—has been converted to a Kozak consensus translation initiation site (2) to increase the translation efficiency in eukaryotic expression systems. The pUC backbone of pZsYellow provides a high-copy-number origin of replication (pUC ori) and an ampicillin resistance gene (Ampr) for propagation and selection in E. coli.

载体应用

pZsYellow Vector serves as a convenient source of the ZsYellow cDNA. The flanking MCS regions make it possible to excise the ZsYellow coding sequence and insert it into other prokaryotic or eukaryotic expression vectors. Alternatively, the ZsYellow coding sequence can be amplified by PCR.

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