
The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS upstream of the Sce VMA intein. The NdeI site in the MCS has the initiator sequence ATG that enables translation of the ORF. The chitin-binding domain(CBD) downstream of the intein results in a target protein-intein with C-terminal chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.
基因搜项目发展历程最早可追溯至2010年01月05日域名注册,项目发起即为促进基因资源共享,避免在基因研究工作中“重复造轮子”,节约广大科研精英的宝贵时间和精力。
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