pcDNA3.1(-)哺乳动物表达载体

载体订购信息

产品编号

GS-1010

产品名称

pcDNA3.1(-) 哺乳动物表达载体

市场价格

询价

沟通洽谈

载体基本信息

载体名称:

pcDNA3.1(-)

载体类型:

哺乳动物表达载体

克隆方法:

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拷贝类型:

高

载体抗性:

Ampicillin (氨苄青霉素)

筛选标记:

Neomycin/G418

载体大小:

5427 bp点击查看完整序列

启动子:

CMV

测序引物5’:

pcDNA3.1-F:CTAGAGAACCCACTGCTTAC

测序引物3’:

pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG

报告基因:

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是否基因产品载体:

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基因产品子货号:

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基因产品规格:

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基因产品货期:

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载体说明

pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: ? Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells ? Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning ? Neomycin resistance gene for selection of stable cell lines ? Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.