pcDNA3.1/Zeo(-)哺乳动物表达载体

载体订购信息

产品编号

GS-1977

产品名称

pcDNA3.1/Zeo(-) 哺乳动物表达载体

市场价格

询价

沟通洽谈

载体基本信息

载体名称:

pcDNA3.1/Zeo(-)

载体类型:

哺乳动物表达载体

克隆方法:

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拷贝类型:

高

载体抗性:

Ampicillin (氨苄青霉素)

筛选标记:

Zeocin (博来霉素)

载体大小:

5014 bp点击查看完整序列

启动子:

CMV

测序引物5’:

pcDNA3.1-F:CTAGAGAACCCACTGCTTAC

测序引物3’:

pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG

报告基因:

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基因产品子货号:

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基因产品规格:

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载体说明

This pcDNA?3.1/Zeo(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin? selectable marker and a reverse-orientation multiple cloning site.

The pcDNA?3.1 Expression Vector Family

Three untagged versions of pcDNA?3.1 (available separately), each with a different selectable marker (Geneticin?, Zeocin?, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

Cytomegalovirus (CMV) enhancer-promoter for high-level expression
Large multiple cloning site in either forward (+) or reverse (-) orientations
Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
Ampicillin resistance gene and pUC origin for selection and maintenance in?E. coli