pFLAG-CMV-3哺乳动物表达载体

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载体订购信息

产品编号
GS-1069
产品名称
pFLAG-CMV-3 哺乳动物表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pFLAG-CMV-3
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (新霉素)
载体大小:
6331 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
CMV-F: CGCAAATGGGCGGTAGGCGTG ; ?hGH-pA-R: CCAGCTTGGTTCCCAATAGA
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pFLAG-CMV?-3 Expression Vector is a 6.3 kb vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.

The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.

The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.

The FLAG? epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.

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