pGEM-7Zf(+)原核表达载体

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载体订购信息

产品编号
GS-1887
产品名称
pGEM-7Zf(+) 原核表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pGEM-7Zf(+)
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
2997 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pGEM?-7Zf(+) and pGEM?-7Zf(–) Vectors are derivatives of the pGEM?-3Zf(+) Vector and contain the origin of replication of the filamentous phage f1. These plasmids serve as standard cloning vectors, as templates for in vitro transcription and can be used for the production of circular ssDNA. These plasmids contain SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate?E. coli?strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI,?ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base? System. pGEM?-7Zf(+) and pGEM?-7Zf(–) Vectors are identical except for the orientation of the f1 origin.

Features - Benefits

  • Blue/White Screening:?Allows the easy identification of recombinant clones.
  • Versatile:?These standard cloning vectors are equipped for single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
  • Convenient:?Multiple cloning site provides a selection of restriction sites for cloning.
  • Unidirectional Deletions:?Restriction sites are positioned conveniently for use with the Erase-a-Base? System.

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