pLVTHRNAi载体

载体订购信息

产品编号

GS-1411

产品名称

pLVTH RNAi载体

市场价格

询价

沟通洽谈

载体基本信息

载体名称:

pLVTH

载体类型:

RNAi载体

克隆方法:

/

拷贝类型:

高

载体抗性:

Ampicillin (氨苄青霉素)

筛选标记:

/

载体大小:

/

启动子:

EF-1 alpha

测序引物5’:

EF-1a Forward: TCAAGCCTCAGACAGTGGTTC

测序引物3’:

/

报告基因:

/

载体图谱展示

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载体说明

This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA. For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid). Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning. Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M et al. (J Virol. 2003 Aug . 77(16):8957-61.