pLVX-EF1α-IRES-mCherry慢病毒载体

Read More
Thumbnail image

载体订购信息

产品编号
GS-2013
产品名称
pLVX-EF1α-IRES-mCherry 慢病毒载体
市场价格
询价
沟通洽谈

载体基本信息

载体名称:
pLVX-EF1α-IRES-mCherry
载体类型:
慢病毒载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
8904 bp 点击查看完整序列
启动子:
EF1α/ EF1a
测序引物5’:
EF1a Forward: TCAAGCCTCAGACAGTGGTTC
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pLVX-EF1α-IRES-mCherry is an HIV-1-based, lentiviral expression vector designed to simultaneously and constitutively express a protein of interest and the green fluorescent protein mCherry from a bicistronic transcript in mammalian cells. mCherry is a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, respectively.

Simultaneous expression of a protein of interest and mCherry is made possible by the presence of an encephalomyo-carditis virus internal ribosome entry site (IRES; 2) positioned between the multiple cloning site (MCS) and the mCherry gene. The IRES allows a protein of interest and mCherry to be translated from a single bicistronic mRNA. Stable, constitutive expression of the bicistronic transcript is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after vector integration into the host cell genome (3).

pLVX-EF1α-IRES-mCherry contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA (4), leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (5). Finally, pLVX-EF1α-IRES-mCherry also contains a central polypurine tract/central termination sequence

关于我们 About Us

公司简介
项目起源

基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。

团队使命

让基因研究更简单!

团队愿景

您值得信赖的一站式基因研究合作伙伴!

联系我们