pMIR-REPORT LuciferaseRNAi载体

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载体订购信息

产品编号
GS-1399
产品名称
pMIR-REPORT Luciferase RNAi载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pMIR-REPORT Luciferase
载体类型:
RNAi载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Puromycin (嘌呤霉素)
载体大小:
6470 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
Luc-F, M13-F20
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The Ambion? pMIR-REPORT? miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

  • Clone miRNA targets and evaluate miRNA regulation
  • Screen putative miRNA target sequences
  • Includes both reporter and control vectors

The pMIR-REPORT? miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT? Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT? Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

载体应用

pMIR-REPORT? Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT? Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR? miRNA Molecules or Anti-miR? miRNA Inhibitor Molecules, respectively.

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