pQCXIN逆转录病毒载体
pQCXIN Retroviral Vector is a bicistronic expression vector designed to express a target gene along with the neomycin selection marker (1). Upon transfection into a packaging cell line, this vector can transiently express, or integrate and stably express a viral genomic transcript containing the CMV immediate early promoter, gene of interest, IRES and the neomycin resistance gene (Neor). The gene of interest and the neomycin resistance gene are co-translated, via the internal ribosome entry site (IRES), from a bicistronic message in mammalian cells (2, 3).
This vector incorporates unique features including: optimization to remove promoter interference and self-inactivation. The hybrid 5' LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This construct drives high levels of transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (4, 5, 6, 7) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3' LTR enhancer region (U3). During reverse transcription of the retroviral RNA, the inactivated 3' LTR is copied and replaces the 5' LTR, resulting in inactivation of the 5' LTR CMV enhancer sequences. This may reduce the phenomenon known as promoter interference (8) and allow more efficient expression.
Also included in the viral genomic transcript are the necessary viral RNA processing elements including the LTRs, packaging signal (Psi+), and tRNA primer binding site. pQCXIN also contains a bacterial origin of replication and?E. coli?Ampr?gene for propagation and selection in bacteria.
pQCXIN is a self-inactivating retroviral vector that is optimized to give high titers, and minimize the possibility of promoter interference. Expresses a gene of interest with the neomycin resistance gene as a bicistronic transcript. pQCXIN is also supplied with the BD Retro-X? Q Vector Set.
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
让基因研究更简单!
您值得信赖的一站式基因研究合作伙伴!