pRL-null信号通路报告载体

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载体订购信息

产品编号
GS-1567
产品名称
pRL-null 信号通路报告载体
市场价格
￥ 1000
沟通洽谈

载体基本信息

载体名称:
pRL-null
载体类型:
信号通路报告载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
3320 bp 点击查看完整序列
启动子:
/
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pRL-null Vector(a,b) (Figure 1) is intended for use in constructing a control reporter vector that may be used in combination with any experimental reporter vector to cotransfect mammalian cells. All of Promega’s pRL Reporter Vectors contain a cDNA(b) (Rluc) encoding Renilla luciferase, which was originally cloned from the marine organism Renilla reniformis (sea pansy; 1). As described below, the Renilla luciferase cDNA contained within the pRL Vectors has been modified slightly to provide greater utility.

The pRL-null Vector contains no enhancer or promoter elements. Rather, it contains a multiple cloning region upstream of Rluc to allow for the cloning of any desired regulatory element(s) to drive expression of Renilla luciferase. Renilla luciferase is a 36kDa monomeric protein that does not require post-translational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction with a reporter vector containing the firefly luciferase gene, refer to the Dual-Luciferase? Reporter Assay System(c,d) Technical Manual (#TM040).

The pRL Vectors are isolated from a dam–/dcm– E. coli K host strain, allowing digestion with restriction enzymes that are sensitive to dam and dcm methylation.

The GenBank?/EMBL Accession Number for the pRL-null Vector is AF025844.

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