pRL-SV40信号通路报告载体

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载体订购信息

产品编号
GS-1571
产品名称
pRL-SV40 信号通路报告载体
市场价格
￥ 1000
沟通洽谈

载体基本信息

载体名称:
pRL-SV40
载体类型:
信号通路报告载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
3705 bp 点击查看完整序列
启动子:
SV40
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pRL-SV40 Vector(a,b) (Figure 1) is intended for use as an internal control reporter and may be used in combination with any experimental reporter vector to cotransfect mammalian cells. All of Promega’s pRL Reporter Vectors contain a cDNA (Rluc) encoding Renilla luciferase, which was originally cloned from the marine organism Renilla reniformis (sea pansy; 1). As described below, the Renilla luciferase cDNA(b) contained within the pRL Vectors has been modified slightly to provide greater utility.

The pRL-SV40 Vector contains the SV40 enhancer and early promoter elements to provide high-level expression of Renilla luciferase in cotransfected mammalian cells. Renilla luciferase is a 36kDa monomeric protein that does not require post-translational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction with a reporter vector containing the firefly luciferase gene, refer to the Dual-Luciferase ? Reporter Assay System (c,d) Technical Manual (#TM040).

The pRL Vectors are isolated from a dam–/dcm– E. coli K host strain, allowing digestion with restriction enzymes that are sensitive to dam and dcm methylation.

The GenBank?/EMBL Accession Number for the pRL-SV40 Vector is AF025845.

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