pTYB2原核表达载体

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载体订购信息

产品编号
GS-1244
产品名称
pTYB2 原核表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pTYB2
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
7474 bp 点击查看完整序列
启动子:
/
测序引物5’:
T7: TAATACGACTCACTATAGGG
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pTYB2 is an E. coli cloning and expression vector (7474 bp) used in the IMPACT? Kit (NEB #E6901) which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag (1,2). This C-terminal fusion vector is designed for the in-frame insertion of a target gene into the polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa) (1,2). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.

For fusion of the N-terminus of the target protein to the intein tag, use pTYB21 (NEB #N6709) or pTYB22 (NEB #N6710). This vector can be used in conjuction with pTYB22 (NEB #N6710) to test which fusion constructin (N-terminal or C-terminal) maximizes the expression and yield of a target protein (3).

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