pBI-CMV1哺乳动物表达载体
pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Protein expression is driven by one of two constitutively active, minimal human cytomegalovirus promoters, PminCMV1 and PminCMV2, located just upstream of two independent multiple cloning sites (MCS 1 and MCS 2, respectively). To allow propagation and selection in E. coli, the vector also contains a ColE1 origin of replication and an ampicillin resistance gene (Ampr). Use: pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.
pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
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