pBIND哺乳动物表达载体

Read More
Thumbnail image

载体订购信息

产品编号
GS-1652
产品名称
pBIND 哺乳动物表达载体
市场价格
￥ 1000
沟通洽谈

载体基本信息

载体名称:
pBIND
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
6360 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The pBIND Vector is a high-copy plasmid in which the CMV immediate early promoter drives expression of a portion of the yeast GAL4 gene (amino acids 1–147) containing a DNA-binding domain. As with the pACT Vector, the DNAbinding domain sequence is flanked by a chimeric intron, a multiple cloning region, stop codons and an SV40 late polyadenylation region (Figures 4 and 5). The fusion gene region is flanked by T7 and T3 RNA polymerase promoters for the purpose of synthesizing sense and antisense RNA products, respectively. The Renilla luciferase gene on this vector is preceded by the SV40 early promoter and a growth hormone intron. Introns can increase protein expression through mRNA stability and nuclear to cytoplasmic transport effects (9–12). A synthetic polyadenylation sequence resides 3? of the Renilla luciferase gene. The plasmid backbone contains an f1 origin of replication for the production of ssDNA and the β-lactamase gene (Ampr) for selection in E. coli.

关于我们 About Us

公司简介
项目起源

基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。

团队使命

让基因研究更简单!

团队愿景

您值得信赖的一站式基因研究合作伙伴!

联系我们