pBS185 CMV-Cre哺乳动物表达载体
AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACATGATTACGAATTCGAGCTCGGTACCCTCGACCAATTCCTGGCCTTCCTGCTGCTACATCTCAAATATCTTGATTACAGGCAGGACCCACGCTCTTTATCACTTTTTTTTCCATCTTTTTTTTTTTGGATATTTTCTTCGTTTATATTTCAAATGCTATCCCAAAAGTCCCCTATACCCCACCCCTACCCCCACCCCGCTCCCCTACCCACTCACTCCCACTTCTTGGCCCTGGCTTTCCCCTGAACTGGGGCATATAGTTTGCTAGACCAAGGGTCCTCTCTTCCCAGTGATGGCCAACTAGGCCATCTTCTGCTACATACGAAGCTAGATACACGAGTTCAGGGGGTACTGGTTAGTTCAGATTGCTGTTCCACCTATAGGGTTGCAGATCCCTTTAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTGTTTGCTGGGCACTGGCAAAGCCTCACAGGAGACAGCTTTATCAGGGTCCTTTCAGCAAAATCTTGATGGCATATGCAATAGTGTCTGCATTTGGTGGCTGAATAGGAGATGGCCTGGTGACACAGTCACTCCTGTAATCCCAGGTACTTGGGAAGCTGAGGCAGGAGAATCAAAAACTCAAGGTCAGACTGGACTGCAGAATGAGTTCTAGGACAAGGTAAGCAACTTGTGTCAAAACAGCCAAGAAAGCCCCTCTGGAATCTAGTTCAGTGTTAGTCCCTGCCTAGCATACGAGACACCCTATGTTAATTTTCATACCCACCTCCTTATACCCAACAATGTGAGAGGAAACCAGAATCCAAAGAGAATATAAATGATAAGATTTTCTGTGCTTCCAGGATGGGACATCAAGTCTTCCAGGACCACCCTCCCTCCCTCCCTTCCCCTAACACAAGACCTGACCAGGCACACAGCTGTTTTCCATGAGGAAGCTTATGGGATAGCTTGGCTTTACCCAAAGACATTACCCCTCTGTGAGGGCCAGAGGCGGGAGGTAAAGGCACGAGGGGGTCCAAAACCAGATCCCCAGACTATCAAGTCACCCCAGTTTCTTATTGCCAAACCACACCAAGAAAACCAGAGTTAGACTCAAACAGGCTTTTATTATTCACGTACTCGGTAGAAAACGGGGGTTTAGTAAACAGGGTGGAACTGTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGCGCTGTTCGTCACATCAGGCACAGCACGTGCACTTGTCCGCGGCGCCTTTGCAGACACAGCCCTGGGCACATTTGGAGCAGCCCACGGGACAGCAGGAGCAGCAGCCTAGAAAGGAGGAGGAAGAGGTCAGCCTGGCTGAAGACCTCTGCTTCCTGTCCCCAACAGAGAAGACCTCGGTTTTTTTAAATAGTTCTCTGTATAGTTCTGTCCCCGAGACAATACAATGGCCTCCGGGAAGCAAAGGACATTTCTCAGAGCCAGTTTTGTAGGAGTTCCCCGCCCCTAGCCTTAGCCGCCACCCAAGGTGTCCCAACTCACTCTTCTTGCAGGAGGTGCACTTGCAGTTCTTGCAGGCGCAGGAGCTGGTGCAAGTGCAGGAGCCGCCTGGGGAGGAGAAAGAAGACAGCATGAGGGAGGCAGCATTACAGCAGTGGCCAACACCACGAGTCCCGGCTCAGTTCACTAAGTCCTCCTCGGAGCTGCAGGGAGCCTAGCCCCACTTTTCTCCTCACAGGTTAAGTCAGGGATTATGTCTTTGAGTCCCAAGACATAAAGGTCCTTCACCTCTTTCTTAATTCAAGCAATTCACAGTAAGTTTAGTAATGCCTGGGACTCAACCCAGATGTCCCCACTAACTGGGTGATCAGGAACCGGACATTTGCGCGTCTAAATCCTAGCCCCAACCCCGCCAAAGGACCCGTTCGACAATACTCGTAACTAATGAAGGCTTTTGCAAGAGCGGATTCTAGAAAGAACCGGAATGAATCGCCAACTAAAGGTGCCTATTCCCGCCAAGCCTCTACAACTCGGGGATTCCGCTCTGCGGTCCCAACTTGGTATTCTAAAGACCAAGGATCGGGAGTCTTACCGGTGGAGCAGGAGCAGTTGGGGTCCATTCCGAGATCCCTCGACAATTCCAACCTTACCCAAGAGTTCGCCAAACTCAGACATCACTTTAGCAAAACCGCGCCGTGCTTCTTCCTCGGTGGCATTCATCACGAAATGTTCAGCACTACGCATACTTTTGACAGGAAACGCAACGGATATTGAGTCAATATCAGGCATTCTATCGCTCAGCTTTACAGTGACAATGACGGCTGGCGACTGAATATTAGTGCTTACAGACAGCACTACATATTTTCCGTCGATGTTGAAATCCTTTCTCATATGTCACCATAAATATCAAATAATTATAGCAATCATTTACGCGTTAATGGCTAATCGCCATCTTCCAGCAGGCGCACCATTGCCCCTGTTTCACTATCCAGGTTACGGATATAGTTCATGACAATATTTACATTGGTCCAGCCACCAGCTTGCATGATCTCCGGTATTGAAACTCCAGCGCGGGCCATATCTCGCGCGGCTCCGACACGGGCACTGTGTCCAGACCAGGCCAGGTATCTCTGACCAGAGTCATCCTTAGCGCCGTAAATCAATCGATGAGTTGCTTCAAAAATCCCTTCCAGGGCGCGAGTTGATAGCTGGCTGGTGGCAGATGGCGCGGCAACACCATTTTTTCTGACCCGGCAAAACAGGTAGTTATTCGGATCATCAGCTACACCAGAGACGGAAATCCATCGCTCGACCAGTTTAGTTACCCCCAGGCTAAGTGCCTTCTCTACACCTGCGGTGCTAACCAGCGTTTTCGTTCTGCCAATATGGATTAACATTCTCCCACCGTCAGTACGTGAGATATCTTTAACCCTGATCCTGGCAATTTCGGCTATACGTAACAGGGTGTTATAAGCAATCCCCAGAAATGCCAGATTACGTATATCCTGGCAGCGATCGCTATTTTCCATGAGTGAACGAACCTGGTCGAAATCAGTGCGTTCGAACGCTAGAGCCTGTTTTGCACGTTCACCGGCATCAACGTTTTCTTTTCGGATCCGCCGCATAACCAGTGAAACAGCATTGCTGTCACTTGGTCGTGGCAGCCCGGACCGACGATGAAGCATGTTTAGCTGGCCCAAATGTTGCTGGATAGTTTTTACTGCCAGACCGCGCGCCTGAAGATATAGAAGATAATCGCGAACATCTTCAGGTTCTGCGGGAAACCATTTCCGGTTATTCAACTTGCACCATGCCGCCCACGACCGGCAAACGGACAGAAGCATTTTCCAGGTATGCTCAGAAAACGCCTGGCGATCCCTGAACATGTCCATCAGGTTCTTGCGAACCTCATCACTCGTTGCATCGACCGGTAATGCAGGCAAATTTTGGTGTACGGTCAGTAAATTGGACATTTCACACTCAGATAATGGTTTTAAGTAAAGTGTACAGGATCGGCTCTGCCCCTCGAGGGGATCCTCTAGAGTCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCAACATGGCGGTAATGTTGGACATGAGCCAATATAAATGTACATATTATGATATGGATACAACGTATGCAATGGGCCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG
BS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.
基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。
让基因研究更简单!
您值得信赖的一站式基因研究合作伙伴!