pBudCE4.1哺乳动物表达载体

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载体订购信息

产品编号
GS-1019
产品名称
pBudCE4.1 哺乳动物表达载体
市场价格
￥ 1500
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载体基本信息

载体名称:
pBudCE4.1
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Zeocin (博莱霉素)
筛选标记:
/
载体大小:
4595 bp 点击查看完整序列
启动子:
EF-1α, CMV
测序引物5’:
CMVPro-F: 5'd[TAATACGACTCACTATAGGG]
测序引物3’:
3'EF1aPro-F:5'd[TCAAGCCTCAGACAGTGGTTC]3'
报告基因:
/

载体图谱展示

载体图谱

载体说明

The pBudCE4.1 vector is designed for the independent expression of two genes from a single plasmid in mammalian cells. Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in the cell. This can eliminate variable expression due to differences in gene copy number. pBudCE4.1 provides expression cassettes withthe following features:

  • The CMV promoter for high-level transcription of genes with an optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification
  • The human EF-1α promoter for high-level expression of genes with an optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
  • The Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E. coliwith the selection agent Zeocin?

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