pcDNA3.1/His B哺乳动物表达载体

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载体订购信息

产品编号
GS-1975
产品名称
pcDNA3.1/His B 哺乳动物表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pcDNA3.1/His B
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (G418)
载体大小:
5515 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
测序引物3’:
pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
报告基因:
/

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载体说明

pcDNA3.1/His A, B, and C are 5.5 kb vectors derived from pcDNA3.1 and designed for high-level expression and purification of recombinant proteins in mammalian hosts. The vectors are supplied in three reading frames to facilitate in frame cloning with a polyhistidine metal-binding tag. The human cytomegalovirus (CMV) immediate-early promoter provides high-level expression in a wide range of mammalian cells. In addition, the vector replicates episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/His lacZ, is the pcDNA3.1/His B vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the N-terminal peptide. pcDNA3.1/His lacZ is included for use as a positive control for transfection, expression, and purification in the cell line of choice.

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