pcDNA3.1/Hygro(-)哺乳动物表达载体

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载体订购信息

产品编号
GS-1980
产品名称
pcDNA3.1/Hygro(-) 哺乳动物表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pcDNA3.1/Hygro(-)
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
/
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Hygromycin (潮霉素)
载体大小:
5596 bp 点击查看完整序列
启动子:
CMV
测序引物5’:
T7 Fwd:5'-TAATACGACTCACTATAGGG-3'
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

This pcDNA?3.1/Hygro(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Hygromycin selectable marker and a reverse-orientation multiple cloning site.

The pcDNA?3.1 Expression Vector Family
Three untagged versions of pcDNA?3.1 (available separately), each with a different selectable marker (Geneticin?, Zeocin?, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

  • Cytomegalovirus (CMV) enhancer-promoter for high-level expression
  • Large multiple cloning site in either forward (+) or reverse (-) orientations
  • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
  • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
  • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

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