pEF4/V5-His A哺乳动物表达载体

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载体订购信息

产品编号
GS-1040
产品名称
pEF4/V5-His A 哺乳动物表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pEF4/V5-His A
载体类型:
哺乳动物表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Zeocin
载体大小:
5769 bp 点击查看完整序列
启动子:
EF-1a
测序引物5’:
T7 Fwd :?5'd[TAATACGACTCACTATAGGG]3'
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pEF4/V5-His A, B, and C are 5.7 kb vectors derived from pEF1/V5-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain thefollowing elements:

  1. Human elongation factor 1a-subunit promoter (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996)(Mizushima and Nagata, 1990).
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide.
  3. Zeocin resistance gene for selection of stable cell lines (Mulsant et al., 1988).
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen(e.g. COS7)

The control plasmid, pEF4/V5-His/lacZ is included for use as a positive control for transfection, expression, purification, and detection in the cell line of choice.

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