pET-32 EK/LIC原核表达载体

Read More
Thumbnail image

载体订购信息

产品编号
GS-1949
产品名称
pET-32 EK/LIC 原核表达载体
市场价格
￥ 1000
沟通洽谈

载体基本信息

载体名称:
pET-32 EK/LIC
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
5917 bp 点击查看完整序列
启动子:
/
测序引物5’:
T7或者Trx-F
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The pET-32 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with the 109aa Trx?Tag? thioredoxin protein. Using specifically designed primers for amplification and the pET-32 Ek/LIC Cloning Kit (Cat. No. 69076-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Fusion proteins also contain cleavable His?Tag? and S?Tag? sequences for detection and purification. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

关于我们 About Us

公司简介
项目起源

基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。

团队使命

让基因研究更简单!

团队愿景

您值得信赖的一站式基因研究合作伙伴!

联系我们