pET-43.1 EK/LIC原核表达载体

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载体订购信息

产品编号
GS-1942
产品名称
pET-43.1 EK/LIC 原核表达载体
市场价格
￥ 1500
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载体基本信息

载体名称:
pET-43.1 EK/LIC
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
7295 bp 点击查看完整序列
启动子:
/
测序引物5’:
T7: 5'-TAATACGACTCACTATAGGG-3'
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pET-43.1 Ek/LIC vector is prepared for rapid, directional cloning of PCR-amplified DNA for high-level expression of polypeptides fused with N-terminal Nus?Tag?, His?Tag? and S?Tag? sequences. Using specifically designed primers for amplification and the pET-43.1 Ek/LIC Vector Kit (Cat. No. 71072-3), inserts can be efficiently cloned without the need for restriction digestion or ligation. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the COLIDOWN primer (Cat. No. 70845-3). Vector encoded sequence can be completely removed when cloning into the Ek/LIC site (as shown below left) by cleaving the fusion protein with enterokinase.

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