载体订购信息

产品编号
GS-1237
产品名称
pETDuet-1 原核表达载体
市场价格
¥ 1000
沟通洽谈

载体基本信息

载体名称:
pETDuet-1
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
5420 bp 点击查看完整序列
启动子:
/
测序引物5’:
T7: 5'-TAATACGACTCACTATAGGG-3';
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱载体图谱

载体说明

pETDuet-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be used in combination with pACYCDuet?-1 (Cat. No. 71147-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the pET Upstream Primer (Cat. No. 69214-3) and DuetDOWN1 Primer (Cat. No. 71179-3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).

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