pET-33b(+)原核表达载体

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载体订购信息

产品编号
GS-1948
产品名称
pET-33b(+) 原核表达载体
市场价格
￥ 1000
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载体基本信息

载体名称:
pET-33b(+)
载体类型:
原核表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Kanamycin (卡那霉素)
筛选标记:
/
载体大小:
5383 bp 点击查看完整序列
启动子:
/
测序引物5’:
T7
测序引物3’:
/
报告基因:
/

载体图谱展示

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载体说明

The pET-33b(+) vector (Cat. No. 69054-3) is derived from pET-28b(+) and carries a 15bp sequence encoding the protein kinase A (PKA) site RRASV, located between the thrombin cleavage and Nde I sites (1). Proteins expressed in pET-33b(+) can be easily purified by metal chelation chromatography (via either N- or C-terminal His?Tag? sequences) and efficiently labeled with 32P- or 33P-gATP and the catalytic subunit of cAMP-dependent protein kinase from heart muscle. Labeled proteins can be used as direct probes in protein-protein interaction studies. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/ expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).

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