pIEX/Bac-1昆虫细胞表达载体

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载体订购信息

产品编号
GS-1717
产品名称
pIEX/Bac-1 昆虫细胞表达载体
市场价格
￥ 1200
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载体基本信息

载体名称:
pIEX/Bac-1
载体类型:
昆虫细胞表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
6796 bp 点击查看完整序列
启动子:
IE1, p10
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The dual-purpose pIEx/Bac? vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep?Tag II coding sequence (1) followed by a recognition site for enterokinase.

The multiple cloning region is followed by an optional C-terminal His?Tag? coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.

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