pIEX/Bac-3昆虫细胞表达载体

Read More
Thumbnail image

载体订购信息

产品编号
GS-2082
产品名称
pIEX/Bac-3 昆虫细胞表达载体
市场价格
￥ 1200
沟通洽谈

载体基本信息

载体名称:
pIEX/Bac-3
载体类型:
昆虫细胞表达载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
/
载体大小:
6802 bp 点击查看完整序列
启动子:
IE1,p10
测序引物5’:
/
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

The pIEx/Bac? vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His?Tag? coding sequence with 10 histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal thrombin protease cleavage site and 8 aa Strep?Tag? II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The 3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

关于我们 About Us

公司简介
项目起源

基因搜项目发展历程最早可追溯至2010年01月05日域名注册，项目发起即为促进基因资源共享，避免在基因研究工作中“重复造轮子”，节约广大科研精英的宝贵时间和精力。

团队使命

让基因研究更简单!

团队愿景

您值得信赖的一站式基因研究合作伙伴!

联系我们