pLVX-EF1α-AcGFP1-N1慢病毒载体

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载体订购信息

产品编号
GS-2008
产品名称
pLVX-EF1α-AcGFP1-N1 慢病毒载体
市场价格
￥ 2200
沟通洽谈

载体基本信息

载体名称:
pLVX-EF1α-AcGFP1-N1
载体类型:
慢病毒载体
克隆方法:
/
拷贝类型:
高
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Puromycin (嘌呤霉素)
载体大小:
9519 bp 点击查看完整序列
启动子:
EF1α/ EF1a
测序引物5’:
EF1a Forward: TCAAGCCTCAGACAGTGGTTC
测序引物3’:
/
报告基因:
/

载体图谱展示

载体图谱

载体说明

pLVX-EF1α-AcGFP1-N1 is an HIV-1-based, lentiviral expression vector designed to constitutively express a protein of interest fused to the N-terminus of AcGFP1, a green fluorescent protein derived from Aequorea coerulescens. The excitation and emission maxima of native AcGFP1 are 475 nm and 505 nm, respectively. Stable, constitutive expression of the fusion protein is driven by the EF1α promoter (PEF1α), which continues to be constitutively active even after the vector integrates into the host cell genome (1).

pLVX-EF1α-AcGFP1-N1 contains all of the viral processing elements necessary for the production of replicationincompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA (2), leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). Finally, pLVX-EF1α-AcGFP1-N1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (4).

In addition to lentiviral elements, pLVX-EF1α-AcGFP-N1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

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