pTrc-CKS原核表达载体

载体基本信息

载体说明

pTrc-CKS表达载体采用E.coliTrc启动子和蛋白质工程改造过CKS (CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase)基因融合表达，同时选用人鼻病毒14亚型3C蛋白酶专一性切割位点。CKS融合表达是美国Abbot公司的专利，CKS融合蛋白研制成功HIV和HCV重组抗原并取得FDA认证在美国上市。CKS是一个非常稳定且高度可溶的理想融合伴侣。本发明除去掉CKS C端8个氨基酸外，还将C端Met突变成Thr

CKS^mut(E.coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase or CMP-KDO synthetase)编码氨基酸序列如下(含多克隆位点)：

MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAERIIVATDHEDVARAVEAAGGEVCMTRADHQSGTE
RLAEVVEKCAFSDDTVIVNVQGDEPMIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSR
ATIPWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIETLEQLRVLWYGEKIHVAVAQEVPGTGVDTP
EDGSMPDSLEVLFQGPAGSAEFSASHHHHHH

268 Amino Acids??? ?Molecular Weight 29KD